Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31.

By A. Groth, M. Fish, R. Nusse, M. Calos. Genetics, 2004

1,101 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/5ace628f40fe30ecfad5a69ab56aff3748650f33

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Citations in the corpus

A GAL4-driver line resource for Drosophila neurobiology. (2012, positive): “These putative enhancer fragments were cloned into a vector containing the GAL4 coding region and a core promoter and then each construct was integrated into the same location in the ﬂy genome by site-speciﬁc integration (Groth et al., 2004).”
Enhancer control of transcriptional bursting (2016, positive): “Transgeneswere integrated into specific genomic locations via phiC31-mediated transgenesis (Groth et al., 2004; Venken et al., 2006).”
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila (2014, methodology): “These plasmids were integrated into the Drosophila genome at defined positions using the attB/attP/Phi31C system (18, 19).”
The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing and Mosaic Analysis (2010, neutral): “Q System Resources—Transgenics and Recombinants Generated in This Study Name Chromosome a QUAS reporters QUAS-mCD8-GFP X, 2nd, 3rd QUAS-mtdTomato-3xHA X, 2nd, 3rd QUAS-nucLacZ X, 2nd, 3rd QUAS-FLPo X, 2nd, 3rd, 3rd (attP2 b) QUAS>stop>mCD8-GFP 2nd, 3rd QUAS-shibirets1 X, 2nd, 3rd QUAS>stop>shibirets”
Genetic dissection of neural circuits. (2008, positive): “For instance, the phage FC31 integrase allows a foreign piece of DNA containing an attP site to integrate at the attB site previously inserted at a specific chromosomal location (Groth and Calos, 2004; Groth et al., 2004; Bischof et al., 2007).”
The VT GAL4, LexA, and split-GAL4 driver line collections for targeted expression in the Drosophila nervous system (2017, methodology): “Germline transformation of attP2 or attP40 hosts (Groth et al., 2004) was performed either in house or by BestGene Inc.”
Genetic manipulation of genes and cells in the nervous system of the fruit fly (2011, methodology): “These plasmids were then integrated at a specific docking site in the fly genome using the FC31 integrase (Groth et al., 2004; Bischof et al., 2007) and tested for expression, resulting in thousands of GAL4 lines (Pfeiffer et al., 2008).”
Generating Customized Transgene Landing Sites and Multi-Transgene Arrays in Drosophila Using phiC31 Integrase (2015, methodology): “, Topics in Current Genetics (Vol. 23, pp. 211–239).”
Cell-type Specific Labeling of Synapses in vivo through Synaptic Tagging with Recombination (STaR) (2014, methodology): “The modified BAC was introduced into flies as a transgene (Groth et al., 2004).”
Tools for neuroanatomy and neurogenetics in Drosophila (2008, methodology): “We used the phiC31 site-specific integration system (11) to insert our constructs in the same orientation at the same genomic location.”
DNA regions bound at low occupancy by transcription factors do not drive patterned reporter gene expression in Drosophila (2012, background): “5-kb sequences were placed upstream of a universal promoter/GAL4 reporter gene fusion, and the resulting constructs were each integrated at a common AttP chromosomal integration site to eliminate position effect variability between transformed lines (30).”