Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

By S. Gratz, Alexander M. Cummings, Jennifer N. Nguyen, Danielle C Hamm, Laura K Donohue, Melissa M. Harrison, Jill Wildonger, Kate M. O’Connor-Giles. Genetics, 2013

1,067 citations recorded by the source corpus.

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

Subfields: Insect Science, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/b8a1f2f80b60f149987585aa3f35073993dbb535

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Citations in the corpus

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA (2014, background): “2013), model organisms (Cho et al. 2013b; Dickinson et al. 2013; Friedland et al. 2013; Gratz et al. 2013; Hwang et al. 2013; Li et al. 2013a,c; Wang et al. 2013; Yang et al. 2013), plants (Li et al.”
Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice (2013, positive): “in bacteria (30,32), yeast (33), zebrafish (34–36), fruit fly (37) and human cells (28,29,38)].”
Loss-of-function approaches in comparative physiology: is there a future for knockdown experiments in the era of genome editing? (2019, methodology): “, 2013), this technique was adopted for studies on fruit flies (Bassett et al., 2013; Gratz et al., 2013), zebrafish (Chang et al.”
Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila (2014, methodology): “…HDR with dsDNA donors Most of the initial experiments employing the CRISPR/Cas9 system in Drosophila have depended on easily scored phenotypes for identification of targeted events (Bassett et al. 2013; Gratz et al. 2013a; Kondo and Ueda 2013; Ren et al. 2013; Sebo et al. 2013; Yu et al. 2013).”
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins (2014, background): “…successfully used to modify genomes in various species including model organisms (Cho et al. 2013b; Dickinson et al. 2013; Friedland et al. 2013; Gratz et al. 2013; Li et al. 2013a,c; Wang et al. 2013; Sung et al. 2014) and plants (Li et al. 2013b; Nekrasov et al. 2013; Shan et al. 2013),…”
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases (2014, background): “…site-specific DSBs, thereby modifying genomes in a targeted manner in cells and organisms (Chang et al. 2013; Cho et al. 2013a,b; Cong et al. 2013; Ding et al. 2013; Gratz et al. 2013; Hwang et al. 2013; Jiang et al. 2013; Jinek et al. 2013; Mali et al. 2013b; Shen et al. 2013; Wang et al. 2013).”
Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans (2014, background): “…genome editing in a wide range of organisms (Wang et al. 1999; Chiu et al. 2013; Cho et al. 2013; Dicarlo et al. 2013; Friedland et al. 2013; Gratz et al. 2013; Hwang et al. 2013; Jiang et al. 2013; Katic and Großhans 2013; Li et al. 2013; Lo et al. 2013; Nekrasov et al. 2013; Kim et al.…”
Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System (2013, background): “The bottom row of the table shows the equivalent numbers from Gratz et al. (2013) for comparison.”