In vitro cultivation of human islets from expanded ductal tissue.

By S. Bonner-Weir, M. Taneja, G. Weir, K. Tatarkiewicz, K. Song, Arun J Sharma, J. O'neil. Proceedings of the National Academy of Sciences of the United States of America, 2000

1,044 citations recorded by the source corpus.

Subfields: Endocrinology, Diabetes and Metabolism, Pharmacology, Surgery

View Paper: https://www.semanticscholar.org/paper/f53c3d0bac1345a30d4018ba74430efe2ca33121

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Surgery

1. Hans Clevers
2. Guoqiang Gu
3. Jolanta Dubauskaite
4. D. Melton

Citations in the corpus

• In vivo derivation of glucose-competent pancreatic endocrine cells from bone marrow without evidence of cell fusion. (2003, background): “Multipotent stem cells have been described within pancreatic islets (11, 12) and in nonendocrine compartments of the pancreas (9, 13), and these cells have the capacity to differentiate into pancreatic islet-like structures in vitro.”
• Insulin production by human embryonic stem cells. (2001, background): “Recently, islet cells were successfully generated in vitro from pancreatic stem cells of humans and adult mice (41,42).”
• Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis (2013, positive): “Culture media was based on AdDMEM/F12 (Invitrogen) supplemented with B27 (Invitrogen), 1.25mM N -Acetylcysteine (Sigma), 10nM gastrin (Sigma) and the growth factors: 50ng/ml EGF (Peprotech), 10% RSPO1-conditioned media (kindly provided by Calvin Kuo), 100ng/ml Noggin (Peprotech) or 10% Noggin-condit”
• Multilineage Differentiation from Human Embryonic Stem Cell Lines (2001, methodology): “Possible methods of achieving this include: A) adding specific combinations of chemical morphogens or growth factors [43, 50 ]; B) co-culturing or co-transplanting ES cells with inducer tissues or cells; C) implanting ES cells into specific organs or regions of animals; D) overexpressing tissue-spe”
• Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. (2002, methodology): “The advantage of using the CRE-ERTM version for lineage analysis is that this protein remains in the cytoplasm and is inactive until a ligand, tamoxifen (TM), is provided (Metzger et al., 1995; Danielian et al., 1998).”
• β Cells Can Be Generated from Endogenous Progenitors in Injured Adult Mouse Pancreas (2008, background): “Long-term culture of heterogeneous populations of pancreas cells favors enrichment of beta (b) cell-like phenotypes (Bonner-Weir et al., 2000; Seaberg et al., 2004; Suzuki et al., 2004) that under certain conditions were able to reverse hyperglycemia when transplanted in diabetic mice (Hao et al.”
• Multipotential nestin-positive stem cells isolated from adult pancreatic islets differentiate ex vivo into pancreatic endocrine, exocrine, and hepatic phenotypes. (2001, background): “(47), all of which were derived from ducts.”