The impact of microRNAs on protein output

By Daehyun Baek, J. Villén, Chanseok Shin, F. Camargo, Steven P. Gygi, David P. Bartel. Nature, 2008

3,798 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f2423a2333d19002cfaff45a9959e922aca7bfa7

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Citations in the corpus

Assessing the ceRNA hypothesis with quantitative measurements of miRNA and target abundance. (2014, background): “The human genome has more than 500 miRNA genes, with miRNAs from individual gene families able to target hundreds of different messenger RNAs (Baek et al., 2008; Friedman et al., 2009).”
MicroRNA regulation by RNA-binding proteins and its implications for cancer (2011, background): “However, although inhibition of translation results in decreased protein levels, the changes in target protein levels following miRNA binding are found to mainly coincide with actual destabilization of the target mRN”
Posttranscriptional regulation of microRNA biogenesis in animals. (2010, background): “Since then, thousands of miRNAs have been found in animals, plants, and unicellular eukaryotes, and it is becoming clear that miRNAs have a big impact on shaping transcriptomes and proteomes of eukaryotic organisms (Baek et al., 2008; Lim et al., 2005; Selbach et al., 2008).”
The Functions of MicroRNAs: mRNA Decay and Translational Repression. (2015, methodology): “Comparing changes at the mRNA and protein levels of potential miRNA targets by using SILAC (Stable Isotope Labeling using Amino acids in cell Culture) or pulsed SILAC coupled with mass spectrometry after expression or deletion of individual mammalian miRNAs revealed that protein changes generally co”
MicroRNAs: the fine-tuners of Toll-like receptor signalling (2011, background): “Each highly conserved mammalian miRNA probably targets several hundred distinct mRNA”
Metazoan MicroRNAs (2018, background): “…examined, mRNA decay dominates, as revealed by experiments comparing changes in mRNA levels with changes in either protein levels or translational efficiency after either introducing or knocking out a miRNA (Baek et al., 2008; Hendrickson et al., 2009; Guo et al., 2010; Eichhorn et al., 2014).”
miR-24 inhibits cell proliferation by suppressing expression of E2F2, MYC and other cell cycle regulatory genes by binding to “seedless” 3′UTR microRNA recognition elements (2009, background): “However, miRNAs in mammals also cause mRNA decay (Chang et al., 2007; Lim et al., 2005; Johnson et al., 2007); recent reports (Baek et al., 2008; Selbach et al., 2008) suggest that reduced protein is frequently associated with decreased mRNA. miR-24 is consistently upregulated during terminal…”
MicroRNAs and their targets: recognition, regulation and an emerging reciprocal relationship (2012, background): “This can be accomplished on a genome-wide scale through transcriptome profiling using microarrays or RNA sequencing (RNA-seq) and through quantitative proteomic platforms, such as stable isotope labelling with amino acids in culture (SILAC”
Mechanisms of miRNA-Mediated Gene Regulation from Common Downregulation to mRNA-Specific Upregulation (2014, background): “It is becoming clear that they have a big impact on shaping transcriptomes and proteomes of eukaryotes [21].”
Functional microRNA targets in protein coding sequences (2012, neutral): “This leads either to translational repression and/or messenger RNA (mRNA) degradation (Bartel, 2009).”
Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. (2010, methodology): “For each set of messages with the indicated 39 UTR site for miR-233 (major isoform sites, bottom left; minor isoform sites, bottom right), the fraction that changed at least to the degree indicated following loss of miR-223 is plotted, using data published for neutrophils differentiated in vivo (Bae”
microRNA-133a regulates cardiomyocyte proliferation and suppresses smooth muscle gene expression in the heart. (2008, background): “Because miRNAs typically diminish the expression of their mRNA targets (Baek et al. 2008; Selbach et al. 2008), we focused on those mRNAs that were up-regulated in dKO hearts, a subset of which would be expected to be direct targets of miR-133a.”
Many roads to maturity: microRNA biogenesis pathways and their regulation (2009, background): “Proteomic studies have recently uncovered the broad impact of a single miRNA on hundreds of target”
Expanding the MicroRNA Targeting Code: Functional Sites with Centered Pairing (2010, methodology): “…or deleting an endogenous miRNA leads to modest yet detectable changes in the output of hundreds of genes containing seed sites in their 3’UTRs (Krutzfeldt et al., 2005; Lim et al., 2005; Giraldez et al., 2006; Grimson et al., 2007; Rodriguez et al., 2007; Baek et al., 2008; Selbach et al., 2008).”
Regulation of mRNA translation and stability by microRNAs. (2010, background): “Although miRNA-mediated deadenylation and subsequent mRNA decay appear to be widespread events (114, 123, 162), not all miRNA-targeted mRNAs are destabilized (for a review, see Reference 5).”