Genome editing with engineered zinc finger nucleases

By F. Urnov, E. Rebar, M. Holmes, H. Zhang, Philip D. Gregory. Nature reviews genetics, 2010

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CRISPR-Cas9 mediated mutagenesis of a DMR6 ortholog in tomato confers broad-spectrum disease resistance (2016, methodology): “NHEJ repair can cause insertions or deletions (indels) around the DNA breaks, whereas HR can repair double-strand breaks using a homologous repair template (Joung and Sander, 2013; Liu and Fan, 2014; Urnov et al. , 2010).”
Improved Modeling of Side-Chain–Base Interactions and Plasticity in Protein–DNA Interface Design (2012, neutral): “9 Promising platforms for generation of genome-speci ﬁ c cleavage reagents are zinc-ﬁ nger nucleases, 10 TALE nucleases, 11 and homing endonucleases or mega-nucleases.”
RNA-programmed genome editing in human cells (2013, background): “…(ZFNs) and transcription activator-like effector nucleases (TALENs) have proved effective for such genomic manipulation but their use has been limited by the need to engineer a specific protein for each dsDNA target site and by off-target activity (Urnov et al., 2010; Bogdanove and Voytas, 2011).”
mRNA-based therapeutics: powerful and versatile tools to combat diseases (2022, background): “mRNA-based stem cell therapeutics: mRNA-based genome editing has also been successfully applied to stem cells for many disease treatments.(486) Previously, ZFN protein, mRNA, and DNA were delivered to a human cell line and mouse embryonic stem cells via a retrovirus vector and disrupted the targeted”