Methods for monitoring autophagy.

By N. Mizushima. International Journal of Biochemistry and Cell Biology, 2004

966 citations recorded by the source corpus.

Subfields: Biochemistry, Cell Biology, Epidemiology

View Paper: https://www.semanticscholar.org/paper/f0fc0f15728c8f2fb59e1cd390d3f891be010ffc

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Citations in the corpus

Discovery of drug mode of action and drug repositioning from transcriptional responses (2010, methodology): “To verify the effect of Fasudil on the induction of autophagic pathway, we evaluated the LC3-II levels in wild-type human fibroblasts treated with Fasudil , by WB with anti-LC3 antibody, a well-established assay for the activation of autophagy (44).”
Small molecules enhance autophagy and reduce toxicity in Huntington's disease models. (2007, methodology): “Although these SMIRs and SMERs were discovered as modulators of the effects of rapamycin on yeast growth, we tested if they were also modulators of mammalian autophagy.”
Lithium induces autophagy by inhibiting inositol monophosphatase (2005, methodology): “Because LC3 specifically associates with autophagosomes, the numbers of LC3-positive vesicles reflect autophagosome number (Kabeya et al., 2000), which is a specific and sensitive assay that has been used widely (for review see Mizushima, 2004).”
The dynamic nature of autophagy in cancer. (2011, methodology): “The measurement of autophagy has been a topic of intense discussion, and there are several comprehensive reviews on the subject as well a variety of published guidelines (Mizushima 2004; Klionsky et al. 2008; Mizushima et al. 2010).”
Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes (2006, background): “S3), resulted in a modest decrease in the conversion of GFP-LC3-I by lipidation to GFP-LC3-II during starvation – a robust read-out for autophagy (Mizushima, 2004) (Fig.”
Protein turnover via autophagy: implications for metabolism. (2007, methodology): “A wide range of methods exist for monitoring autophagy in yeasts, and several approaches can be used in higher eukaryotes; these have been covered in various reviews (43, 47, 67).”
Novel targets for Huntington’s disease in an mTOR-independent autophagy pathway (2008, methodology): “Clonidine, minoxidil and verapamil increase autophagy We assessed autophagosome numbers using the microtubule-associated protein 1 light chain 3 (LC3”
Role of autophagy in cancer (2007, background): “ATG8 (also known as MAP1LC3 ) is another ubiquitin-like protein that is cleaved, lipidated and becomes a component of the autophagosome membrane, and this membrane translocation event is commonly used to monitor autophag”