Exploration of essential gene functions via titratable promoter alleles.
By S. Mnaimneh, A. Davierwala, J. Haynes, Jason Moffat, W. Peng, Wen Zhang, Xueqi Yang, Jeff Pootoolal, Gordon Chua, Andrés López and 14 more. Cell, 2004
650 citations recorded by the source corpus.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/ea3838e4a932d25afb7eb49ab819dab2c26f6377
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In Molecular Biology
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Citations in the corpus
- Genetic reconstruction of a functional transcriptional regulatory network (2007, methodology): “For the essential transcription factor HSF1 , we used a conditional shut-off strain 12 .”
- A global interaction network maps a wiring diagram of cellular function (2016, result): “First, consistent with the results of our previous smaller-scale surveys (1, 24), essential genes are major hubs and form the basic scaffold of the global genetic interaction network.”
- Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile. (2005, methodology): “DAmP utilizes simple, PCR-based homologous recombination to disrupt the natural 3#UTR by insertion of an antibiotic-resistance marker, thus greatly destabilizing the corresponding messages (Muhlrad and Parker, 1999).”
- Mapping pathways and phenotypes by systematic gene overexpression. (2006, methodology): “The genetic accessibility of the budding yeast S. cerevisiae has allowed the development of genome-wide loss-of-function mutant collections , including gene deletion (Giaever et al., 2002) and titratable promoter alleles (Mnaimneh et al., 2004), for systematic scrutiny of gene function.”
- Progress in Metabolic Engineering of Saccharomyces cerevisiae (2008, methodology): “Recently the bacterial tet promoter system has been used for inducer-triggered repression of all essential genes in yeast (225, 402).”
- Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double strand break ends (2008, positive): “Instead we constructed DNA2 under the regulatable TetO 7 promoter in which DNA2 expression is shut off by the addition of doxocycline to the growth media (Mnaimneh et al., 2004).”