17-P022 Virus-free induction of pluripotency and subsequentexcision of reprogramming factors

By K. Kaji, K. Norrby, Agnieszka Paca, M. Mileikovsky, P. Mohseni, K. Woltjen. 2009

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Citations in the corpus

Senescence impairs successful reprogramming to pluripotent stem cells. (2009, background): “2008), transposons (Kaji et al. 2009), adenoviral (Stadtfeld et al.”
A Small Molecule Inhibitor of Tgf-β Signaling Replaces Sox2 in Reprogramming by Inducing Nanog (2009, background): “Identification of such compounds would allow reprogramming that would not be impeded by the laborious nature of protein transduction or the safety concerns surrounding transgenic approaches (Kaji et al., 2009; Kim, 2009; Okita et al., 2008).”
Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation (2009, background): “Recent studies on iPS clones generated with excisable vector systems demonstrate that residual exogenous factor expression does not hinder high-level chimeric contribution (11), although it perturbs the transcriptional profile (33) or the differentiation ability (10) of iPSCs.”
Risk factors in the development of stem cell therapy (2011, background): “Consequently the negative effect related to the integration of the reprogramming factors is prevented [72-74].”
In vivo direct reprogramming of reactive glial cells into functional neurons after brain injury and in an Alzheimer’s disease model (2013, background): “…or small-molecule strategy to effect the reprogramming in vivo (Bayart and Cohen-Haguenauer, 2013; Chambers et al., 2012; Hou et al., 2013; Kaji et al., 2009; Li et al., 2013; Cell Stem Cell 14, 1–15, February 6, 2014 ª2014 Elsevier Inc. 9 (A) The majority of cultured human astrocytes…”
Reprogramming of mouse and human cells to pluripotency using mature microRNAs. (2011, background): “Most of the reprogramming methods reported so far involve introduction of genetic materials and thus run the risk that exogenous vector sequences can be integrated into the host genome (Okita et al., 2008; Kaji et al., 2009; Woltjen et al., 2009; Jia et al., 2010).”
Complexity in biomaterials for tissue engineering. (2009, background): “The latest report achieved reprogramming in human cells with no permanent genetic modificatio”
A fresh look at iPS cells. (2009, methodology): “More recently, iPS cells have been generated by genomic integration of the four reprogramming factors using plasmids (Kaji et al., 2009), lentiviruses (Soldner et al., 2009), or transposons (Woltjen et al., 2009), followed by transgene removal using Cremediated excision or re-expression of…”
Induced pluripotency: history, mechanisms, and applications. (2010, background): “To avoid this issue, several laboratories have developed integration-dependent gene delivery vectors with incorporated loxP sites that can be subsequently excised from the host genome by transient expression of Cre recombinase (Kaji et al. 2009; Soldner et al. 2009).”
Hypoxia enhances the generation of induced pluripotent stem cells. (2009, background): “It was also reported that MEFs could be reprogrammed by transcription factors delivered by piggyBac (PB) transposition system (Kaji et al., 2009; Woltjen et al., 2009).”