Specific interference with gene function by double-stranded RNA in early mouse development

By F. Wianny, M. Zernicka‐Goetz. Nature Cell Biology, 2000

771 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/d5d5f6f1659574151f8c9b2777aeadbab7345aa5

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In Molecular Biology

Citations in the corpus

Mouse ES cells express endogenous shRNAs, siRNAs, and other Microprocessor-independent, Dicer-dependent small RNAs. (2008, background): “Indeed, the two cell types where a large number of mammalian siRNAs have been unambiguously identified, oocytes (Tam et al. 2008; Watanabe et al. 2008) and mESCs (this study) lack an interferon response ( Wianny and Zernicka-Goetz 2000; Yang et al. 2001).”
Role for a bidentate ribonuclease in the initiation step of RNA interference (2001, background): “Exogenous dsRNAs can affect gene function in early mouse embryo”
Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline. (2001, methodology): “Reactions were incubated for 10 min at 25 (cid:7) C and then 1 hr at 43 (cid:7) C. RT reactions (2 Recent reports of RNAi experiments in mammals [4, 56] (cid:6) l) was used in PCR with the PD2/PR3 primer pair.”
Induction and suppression of RNA silencing: insights from viral infections (2005, background): “Perhaps, the occurrence and effects of vsRNAs will only be evident in vertebrate cells in which the IFN or humoral immune responses are compromised or naturally inactive, as in embryonic stem (ES) cells or pre-implanted embryo”
A Review of Automated Microinjection Systems for Single Cells in the Embryogenesis Stage (2016, methodology): “injecting a newly-fertilized zygote with a DNA solution [11]; 2) performing gene studies by injecting animal blastocysts with modified ES cells [12]; 3) creating clones by transplanting a”
RNA interference is mediated by 21- and 22-nucleotide RNAs. (2001, background): “The drosha-like human RNase III was recently cloned and characterized (Wu et al. 2000).”
Therapeutic EphA2 gene targeting in vivo using neutral liposomal small interfering RNA delivery. (2005, background): “injection (33, 34), others have shown that siRNA has activity in vivo using delivery in viral vectors (8, 9), retinal electroporation (35), and direct intracellular (36), intratumoral (37), intravitreal (38), intranasal (39), and intrathecal (40) administration.”
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells (2001, background): “This apparent lack of RNAi in mammalian cell culture was unexpected, because RNAi exists in mouse oocytes and early embryo”