Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

By Sayda M. Elbashir, J. Harborth, Winfried Lendeckel, A. Yalçin, K. Weber, Thomas Tuschl. Nature, 2001

9,764 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/efd52e2f0692d5aba49c037cd4869e68345b5760

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. David M. Sabatini
2. Weimin He
3. David P. Bartel
4. Phillip D. Zamore
5. John J. Rossi
6. L. Aagaard
7. John S. Mattick
8. R. Weinberg

In Molecular Biology

1. David M. Sabatini
2. David P. Bartel
3. Phillip D. Zamore
4. Thomas Tuschl
5. John S. Mattick
6. Yukihide Tomari
7. Phillip A. Sharp
8. V. Narry Kim

Citations in the corpus

• Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system. (2004, background): “Potential applications of rAAV vectors include their use to overexpress genes [11,12] and to knock down gene expression using antisense, ribozymes, and siRNAs [9,18–21].”
• A large-scale RNAi screen in human cells identifies new components of the p53 pathway (2004, neutral): “In mammalian cells, an RNAi response can be triggered by 21-base-pair siRNAs, which can cause strong, but transient, inhibition of gene expression 7 .”
• ATP requirements and small interfering RNA structure in the RNA interference pathway. (2001, positive): “These results, together with those of phase of the cleavage reaction, when the rate of dsRNA Tuschl and coworkers (Elbashir et al., 2001a), demoncleavage is linear (Figure 1A).”
• Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. (2005, background): “, 2005), both of which are Dicer substrates, are considerably more potent triggers of RNAi than the short duplex siRNA (Elbashir et al., 2001) in transfected cells.”
• Plk4-induced centriole biogenesis in human cells. (2007, methodology): “…(Habedanck et al., 2005), and Myc-Plk4 expression was induced by the addition of 1 mg ml 1 of tetracyclin. siRNA-Mediated Protein Depletion Centrosomal proteins were depleted using siRNA duplex oligonucleotides (see Table S1), using the luciferase duplex GL2 for control (Elbashir et al., 2001).”
• The small RNA profile during Drosophila melanogaster development. (2003, background): “…that are processed by the ribo-Terry Gaasterland,4 Jutta Meyer,2 nuclease type III enzyme Dicer (Bernstein et al., 2001;and Thomas Tuschl2,* Elbashir et al., 2001b; Grishok et al., 2001; Hutvágner et1Department of Animal Molecular Genetics al., 2001; Ketting et al., 2001; Park et al.,…”
• Efficiency of siRNA delivery by lipid nanoparticles is limited by endocytic recycling (2013, neutral): “Small interfering RNAs (siRNA) are a potential new class of medicines that can selectively silence disease-causing genes 1–3 .”
• Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor. (2002, methodology): “This level of inhibition is similar to results from previous studies using luciferase reporter plasmid systems (23,24).”