Next-Generation Optical Technologies for Illuminating Genetically Targeted Brain Circuits

By Karl Deisseroth, G. Feng, Ania Majewska, Gero Miesenböck, Alice Y. Ting, Mark J. Schnitzer. Journal of Neuroscience, 2006

749 citations recorded by the source corpus.

Emerging technologies from optics, genetics, and bioengineering are being combined for studies of intact neural circuits. The rapid progression of such interdisciplinary “optogenetic” approaches has expanded capabilities for optical imaging and genetic targeting of specific cell types. Here we explore key recent advances that unite optical and genetic approaches, focusing on promising techniques that either allow novel studies of neural dynamics and behavior or provide fresh perspectives on classic model systems.

Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience

View Paper: https://www.semanticscholar.org/paper/d30276bc48529bcde00606d992470936518706ff

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Citations in the corpus

Neurophysiological and Computational Principles of Cortical Rhythms in Cognition (2010, background): “Fifth, with the new targeted optogenetic manipuation that allows one to rapidly and selectively activate or d-activate a subtype of nerve cells (227, 411, 613), it is now possible to establish causal links between an observed neural activity pattern and its underlying circuit mechanism(s).”
Neural syntax: cell assemblies, synapsembles and readers (2010, neutral): “Since methods for selective and fast activation and inactivation ofmultiple single neurons and synapses by light activation are on the horizon (Boyden et al., 2005; Deisseroth et al., 2006; Luo et al., 2008; Miesenböck, 2009; Zhang et al., 2007), discussion of their potential use in identifying asse”
An optical neural interface: in vivo control of rodent motor cortex with integrated fiberoptic and optogenetic technology (2007, background): “In this paper we present results showing the successful development and implementation of a novel optical neural interface based on integrated fiberoptic and optogenetic technology [21].”
The cell biology of synaptic plasticity: AMPA receptor trafficking. (2007, background): “The manipulation of neuronal activity has also become easier with the advent of two-photon uncaging of neurotransmitters and second messengers as well as advances in light-activated ion channels (Deisseroth et al. 2006).”
Pyramidal neurons: dendritic structure and synaptic integration (2008, methodology): “These include cell stimulation using glutamate uncaging or optically activated channels and pump”
Multimodal fast optical interrogation of neural circuitry (2007, background): “Author Information The GenBank accession number is EF474018 for the ‘mammalianized’ NpHR sequence and EF474017 for the ‘mammalianized’ ChR2(1-315) sequence.”
The Development and Application of Optogenetics (2011, background): “Building both on our finding that adequate retinal is present within mammalian brain tissue (Deisseroth et al. 2006, Zhang et al. 2006) and on a long history of elegant GPCR structure-function work from Khorana, Kobilka, Caron, Lefkowitz, and others, we have determined that GPCRs can be converted…”
Molecular and cellular approaches for diversifying and extending optogenetics. (2010, background): “To enable temporally precise control of specific cell types within behaving animals, fast ‘‘optogenetic’’ (Deisseroth et al., 2006) technologies have been developed involving singlecomponent light-responsive proteins that transduce brief pulses of light into well-defined action potential trains and…”