The acetyltransferase activity of San stabilizes the mitotic cohesin at the centromeres in a shugoshin-independent manner

By Fajian Hou, Chih-Wen Chu, Xiangduo Kong, K. Yokomori, Hui Zou. Journal of Cell Biology, 2007

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Proper sister chromatid cohesion is critical for maintaining genetic stability. San is a putative acetyltransferase that is important for sister chromatid cohesion in Drosophila melanogaster, but not in budding yeast. We showed that San is critical for sister chromatid cohesion in HeLa cells, suggesting that this mechanism may be conserved in metazoans. Furthermore, although a small fraction of San interacts with the NatA complex, San appears to mediate cohesion independently. San exhibits acetyltransferase activity in vitro, and its activity is required for sister chromatid cohesion in vivo. In the absence of San, Sgo1 localizes correctly throughout the cell cycle. However, cohesin is no longer detected at the mitotic centromeres. Furthermore, San localizes to the cytoplasm in interphase cells; thus, it may not gain access to chromosomes until mitosis. Moreover, in San-depleted cells, further depletion of Plk1 rescues the cohesion along the chromosome arms, but not at the centromeres. Collectively, San may be specifically required for the maintenance of the centromeric cohesion in mitosis.

Subfields: Cell Biology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/cbfa4df36aa471f12618db1d56fb95deffbdd38d

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Citations in the corpus

Modeling semiflexible polymer networks (2014, background): “…elastic rod is embedded in a surrounding elastic material, as can be the case for microtubules embedded in the matrix formed by the rest of the cytoskeleton (Brangwynne et al., 2006), then this classical Euler buckling problem and corresponding threshold is altered (Landau and Lifshitz, 1986).”
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Review on Cell Mechanics: Experimental and Modeling Approaches (2013, background): “They grow dynamically by polymerization at their ends furthest from the MTOC [31] and can resist cellular compressive forces [32].”
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Mechanical control of tissue and organ development (2010, background): “…cytoskeleton are also resisted by these adhesive tethers to neighboring cells and the ECM, and by internal cytoskeletal structures that resist being compressed, such as microtubules or cross-linked actin filaments within filopodia (Wang et al., 2001; Brangwynne et al., 2006; Ingber, 2006).”
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Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus (2009, background): “) This model takes into account the observation that individual cytoskeletal filaments can bear significant tensile and compressive loads in living cells because their structural integrity is maintained for longer than the turnover time of individual protein monomer”