Adult SVZ stem cells lie in a vascular niche: A quantitative analysis of niche cell-cell interactions

By Qin Shen, Yue Wang, Erzsebet Kokovay, Gang Lin, Shu‐Mien Chuang, Susan K. Goderie, B. Roysam, Sally Temple. 2008

1,065 citations recorded by the source corpus.

There is an emerging understanding of the importance of the vascular system within stem cell niches. Here we examine whether neural stem cells (NSCs) in the adult subventricular zone (SVZ) lie close to blood vessels, using 3-dimensional wholemounts, confocal microscopy and automated computer-based image quantification. We found that the SVZ contains a rich plexus of blood vessels that snake along and within neuroblast chains. Cells expressing stem cell markers, including GFAP, and proliferation markers, are closely apposed to the laminin-containing extracellular matrix (ECM) surrounding vascular endothelial cells. Apical GFAP+ cells are admixed within the ependymal layer and some span between the ventricle and blood vessels, occupying a specialized microenvironment. Adult SVZ progenitor cells express the laminin receptor alpha6beta1 integrin, and blocking this inhibits their adhesion to endothelial cells, altering their position and proliferation in vivo, indicating it plays a functional role in binding SVZ stem cells within the vascular niche.

Subfields: Cancer Research, Genetics, Molecular Biology

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Citations in the corpus

Adult Neurogenesis in the Mammalian Brain: Significant Answers and Significant Questions (2011, neutral): “In the adult SVZ, the vasculature comprises an extensive network of planar interconnected blood vessels (Shen et al., 2008; Tavazoie et al., 2008).”
The Glial Nature of Embryonic and Adult Neural Stem Cells (2009, positive): “…indicate that type B cells retain some important properties of RG. Type B cell bodies are generally located just under the ependymal cell layer but have short processes that extend through the ependymal layer with small apical endings on the ventricle (Mirzadeh et al. 2008, Shen et al. 2008).”
Integrin alpha 6 regulates glioblastoma stem cells (2010, positive): “In the adult murine brain, integrin a6 expression has been reported in the neural stem cell compartment adjacent to the lateral ventricles (Shen et al., 2008) and a detailed analysis suggests that the proliferating cells express integrin a6 as do the neural stem cell upon activation (I.”
Extracellular matrix: A dynamic microenvironment for stem cell niche (2014, positive): “which binds the ECM protein laminin, is essential to home spermatogonial stem cells in the testicular niche [46], and is required for the adhesion of neural stem cells (NSCs) to their vascular niche [47].”
Adult Mammalian Neural Stem Cells and Neurogenesis: Five Decades Later (2015, neutral): “, 2015) and SVZ (Shen et al., 2008), and adult NSCs extend processes that are closely associated with blood vessels (Figure 2).”
Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain (2011, neutral): “Although BrdU labeling of mechanical z sections from the subventricular zone (SVZ”
Oxygen in stem cell biology: a critical component of the stem cell niche. (2010, positive): “This regulation is mediated through close contact of type B1 astrocytes with the cerebrospinal fluid and blood vessels (Doetsch et al., 1999; Mirzadeh et al., 2008; Shen et al., 2008; Tavazoie et al., 2008).”
The cerebrospinal fluid provides a proliferative niche for neural progenitor cells (2011, positive): “, 2006), by insulating progenitor cells from proliferative signals in CSF, with vascular niches potentially supplying sources of secreted factors for stem cells at other stages (Palmer et al., 2000; Shen et al., 2004; Shen et al., 2008; Tavazoie et al., 2008).”
Extracellular Matrix Degradation and Remodeling in Development and Disease (2011, neutral): “Such localization reflects an anchorage of neural stem cells, which express a6b1 integrins, on laminin in the vascular basement membrane ECM (Shen et al. 2008).”
Extracellular matrix: a dynamic niche component during cancer progression (2012, positive): “For example, various ECM receptors have been used as markers to enrich adult stem cells in many in vitro and in vivo systems (Shen et al., 2008; Raymond et al., 2009), suggesting that contact with the ECM is necessary for cells to acquire or maintain stem cell properties.”
FoxO3 regulates neural stem cell homeostasis (2009, positive): “NSCs are located in close proximity to blood vessels in vivo and may be subjected to varying levels of oxygen (Mirzadeh et al., 2008; Shen et al., 2008; Tavazoie et al., 2008), so the ability of FoxO3 to regulate oxygen metabolism in NSCs might also play a role in the protection of these cells in vi”
BRAIN REGENERATION IN PHYSIOLOGY AND PATHOLOGY: THE IMMUNE SIGNATURE DRIVING THERAPEUTIC PLASTICITY OF NEURAL STEM CELLS (2011, positive): “Type B cell bodies are generally located just under the ependymal cell layer but have short processes that extend through the ependymal layer with small apical endings that contact the ventricle (150, 215).”
Lake-front property: a unique germinal niche by the lateral ventricles of the adult brain. (2011, positive): “Type B1 cells have several characteristics that are reminiscent of their radial glial progenitors – a thin apical process with a primary cilium extending into the ventricular lumen, a basal process that extends to reach blood vessels, and behavior that is reminiscent of the interkinetic nuclear migr”