Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain

By H. Hama, H. Kurokawa, H. Kawano, R. Ando, T. Shimogori, Hisayori Noda, K. Fukami, A. Sakaue-Sawano, A. Miyawaki. Nature Neuroscience, 2011

1,132 citations recorded by the source corpus.

Subfields: Biophysics, Cellular and Molecular Neuroscience

View Paper: https://www.semanticscholar.org/paper/5faff85a16d13e42932a778649b60f2100b143cd

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In Biophysics

In Cellular and Molecular Neuroscience

Citations in the corpus

iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. (2014, neutral): “Recently, there has been a resurgence of interest in optimizing methods for volume imaging of molecularly labeled structures in large, cleared tissue samples, leading to the development of several important new clearing methods: ScaleA2 (Hama et al., 2011), 3DISCO (Ertürk et al.”
Labkit: Labeling and Segmentation Toolkit for Big Image Data (2021, background): “, 2006), modern tissue clearing (Dodt et al., 2007; Hama et al., 2011), or serial section scanning electron microscopy (Denk andHorstmann, 2004; Knott et al.”
Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis. (2014, positive): “We considered 40 chemicals, including polyhydric alcohols, detergents, and hydrophilic small molecules (Table S1 available online), corresponding to the components of the ScaleA2 solution: glycerol, Triton X-100, and urea.”
The Human Cell Atlas (2017, neutral): “…2016); the distribution of scores of proteins at cellular or sub-cellular resolution (Angelo et al., 2014; Chen et al., 2015a; Giesen et al., 2014; Hama et al., 2011; Susaki et al., 2014; Yang et al., 2014); various aspects of chromatin state (Buenrostro et al., 2015; Cusanovich et al., 2015;…”
BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples (2018, background): “Sample clearing [1, 2] and expansion microscopy (ExM) [3] are powerful protocols that create large, transparent volumes of whole tissues and organisms.”
Single-Cell Phenotyping within Transparent Intact Tissue Through Whole-Body Clearing (2014, positive): “Traditionally, making tissue transparent was a process that demanded solvent incubations on the order of weeks to months, as reported in other clearing protocols (Hama et al., 2011).”
Type 2 alveolar cells are stem cells in adult lung. (2013, positive): “All of these data from confocal analysis of sections were recapitulated when whole-mount lung lobes were examined with a multiphoton microscope after clearing in Sca l e solution (30), thereby enabling imaging deeper into the tissue (Figure 4B and Supplemental Figure 4C).”
Clarifying Tissue Clearing (2015, positive): “While some of the recently published clearing techniques were developed serendipitously (Scale, ClearT), others were intentionally engineered.”