Ring1-mediated ubiquitination of H2A restrains poised RNA polymerase II at bivalent genes in mouse ES cells

By J. Stock, Sara Giadrossi, Miguel Casanova, Emily Brookes, M. Vidal, Haruhiko Koseki, N. Brockdorff, A. Fisher, A. Pombo. Nature Cell Biology, 2007

718 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/c00eec4ee4cd4a97b0c060c8d0e6197af8391d3d

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Citations in the corpus

Control of Embryonic Stem Cell State (2011, background): “…Boyer et al., 2006; Bracken et al., 2006; Lee et al., 2006; Leeb et al., 2010; Li et al., 2010; Pasini et al.; Peng et al., 2009; Shen et al., 2009; Stock et al., 2007; van der Stoop et al., 2008); 23 (Bilodeau et al., 2009; Yeap et al., 2009; Yuan et al., 2009); 24 (Ho and Crabtree, 2010; Schnetz…”
Genome Regulation by Polycomb and Trithorax: 70 Years and Counting. (2017, neutral): “PRC1-mediated H2A ubiquitination was initially suggested to play an important role in PcG-mediated repression by interfering with multiple steps of the transcription process (Nakagawa et al., 2008; Stock et al., 2007; Zhou et al., 2008).”
Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put (2013, neutral): “Does it directly impede a transcription process such as elongation and/or phosphorylation of RNA polymerase II (Brookes et al., 2012; Stock et al., 2007; Zhou et al., 2008)?”
Histone variants — ancient wrap artists of the epigenome (2010, neutral): “RING1B is necessary for repression at these sites 128 .”
Distinct factors control histone variant H3.3 localization at specific genomic regions (2010, methodology): “The CTD4H8 antibody was raised against a chemically synthesized phospho-Ser 5 peptide sequence from the C-terminal domain (CTD) of the largest RPB1 subunit of RNAPII and has been extensively characterized previously (Stock et al., 2007).”
Polycomb group proteins: multi-faceted regulators of somatic stem cells and cancer. (2010, neutral): “In fact, these loci appear to be in a poised state ready for activation since the RNA polymerase II phosphorylated at serine 5, which is associated with paused transcription, also localizes to these loci (Stock et al., 2007).”
Charting histone modifications and the functional organization of mammalian genomes (2011, background): “, subsequent studies have suggested that at least a subset has detectable RNAPII enrichmen”
The Transcriptional and Epigenomic Foundations of Ground State Pluripotency (2012, background): “Attention has also been drawn to colocalization at many promoters of histone 3 lysine 4 trimethylation (H3K4me3), associated with transcriptional activation, and histone 3 lysine 27 trimethylation (H3K27me3), linked with repression (Azuara et al., 2006; Bernstein et al., 2006; Stock et al., 2007).”
Mechanisms of Polycomb gene silencing: knowns and unknowns (2009, background): “Knockout studies imply that H2A ubiquitylation plays a key part in PcG repressio”
Histone ubiquitination: triggering gene activity. (2008, background): “There are some indications that Ring1B and H2A ubiquitination are required to maintain RNA polymerase II in a Ser5-phosphorylated configuration on genes that are poised for, but not actively transcribed in, ES cells (Stock et al., 2007).”
CpG islands and the regulation of transcription. (2011, neutral): “PRC2 mediates H3K27me3, and this mark is recognized by PRC1, which is thought to inhibit transcriptional elongation by a mechanism involving H2A ubiquitylation (Stock et al. 2007; Zhou et al. 2008) and/or chromatin compaction (Eskeland et al. 2010).”
Transcriptional regulation by Polycomb group proteins (2013, background): “; and from the Novo Nordisk Foundation, the Danish Cancer Society and the Danish Council for Strategic Research (12-110503) to K.”
The Polycomb Complex PRC2 and its Mark in Life (2011, background): “RNA polymerase (Pol) II that is phosphorylated at Ser 5 of its C-terminal domain is present in a substantial fraction of H3K27me3-enriched promoter”
Complex multi-enhancer contacts captured by Genome Architecture Mapping (GAM) (2017, neutral): “3f-h), cryoFISH was performed as previously described37,54,55 in mouse ES-OS25 cells (kindly provided by W. Bickmore) grown as previously described32,55, and prepared for cryosectioning as described above.”