Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

By Zhonggang Hou, Yan Zhang, Nicholas E. Propson, S. Howden, Li-Fang Chu, E. Sontheimer, James A. Thomson. Proceedings of the National Academy of Sciences of the United States of America, 2013

657 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/c3c29e47358bbe42719e5728cb163695ff4c30ba

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Citations in the corpus

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases (2014, background): “We and others have developed three different types of engineered nucleases: zinc finger nucleases (ZFNs) (Bibikova et al., 2003; Kim et al., 2009), transcription activator-like effector nucleases (TALENs) (Kim et al., 2013; Miller et al., 2011) and RNA-guided engineered nucleases (RGENs) (Cho et…”
Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites (2015, neutral): “First, Cas-Designer finds possible target sites with a user-defined PAM sequence [5 0 -NGG-3 0 or 5 0 -NRG-3 0 for SpCas9, 5 0 - NNAGAAW-3 0 for StCas9 (Cong et al., 2013), 5 0 -NNNNGMTT-3 0 for NmCas9 (Hou et al., 2013) and 5 0 -NNGRRT-3 0 for SaCas9 (Ran et al., 2015)] in a given DNA sequence.”
Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. (2013, background): “Recently, several groups have demonstrated the use of the CRISPR/Cas9 system for genome engineering in various species (Chang et al., 2013; Cho et al., 2013; Cong et al., 2013; Friedland et al., 2013; Hou et al., 2013; Hwang et al., 2013; Jinek et al., 2013; Li et al., 2013; Mali et al., 2013; Nekra”
Development and Applications of CRISPR-Cas9 for Genome Engineering (2014, positive): “Another Cas9 from Neisseria meningitidis with a 50-NNNNGATT PAM requirement (Zhang et al., 2013) was recently applied in human pluripotent stem cells (Hou et al., 2013).”
The new frontier of genome engineering with CRISPR-Cas9 (2014, positive): “The reported collection of Cas9 orthologs constitutes a large source of CRISPR-Cas9 systems for multiplex gene targeting, and several orthologous CRISPR-Cas9 systems have already been applied successfully for genome editing in human cells [Neisseria meningitidis (73, 74), S.”
Cas-analyzer: an online tool for assessing genome editing results using NGS data (2016, neutral): “Availability and Implementation: Free access at http://www.rgenome.net/cas-analyzer/.”
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation (2014, background): “Although most genome engineering methodologies currently use SpyCas9, there is considerable interest in exploitingmore compact Cas9 enzymes for such applications (21, 22).”