Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

By Li-En Jao, S. Wente, Wenbiao Chen. Proceedings of the National Academy of Sciences of the United States of America, 2013

1,316 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/677170045bca8db625f2d8a3e4ae9be132775d5f

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Citations in the corpus

Reprogramming homing endonuclease specificity through computational design and directed evolution (2013, methodology): “For example, the CRISPR/Cas9 family of nucleases is becoming a popular catalyst for genome engineering (6,49–51), and the methods presented in this work could be used to increase the speciﬁcity of Cas9 (52–54) or expand the targeting potential of this class of enzymes, currently limited by the PAM…”
Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs (2014, result): “This high level of mutagenesis indicates that Cas9 is an effective method for generating targeted mutations in zebrafish, in agreement with previously published results [9,10,12,13,15].”
Disruptive CHD8 mutations define a subtype of autism early in development (2014, methodology): “As a final test of specificity of the phenotype, we induced microdeletions in exon 2 of chd8 by the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system (Hwang et al., 2013; Jao et al., 2013) (Figure S5A) and validated the presence of genetic editing in 50% of the injected ”
Target specificity of the CRISPR-Cas9 system (2014, neutral): “Up to five genes have been mutated simultaneously in mouse and fish cells by delivering five guide RNAs [14,15].”
sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites (2014, methodology): “The CRISPR-Cas9 system has been successfully used in gene targeting of different species, including the monkey, human induced pluripotent stem cells, the mouse, the rat, the zebrafish and the fly [9–22].”
A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, background): “In vertebrates such as zebrafish, mice, rats, and monkeys, coinjection of gRNA and Cas9-encoding mRNA transcribed in vitro into single-cell-stage embryos can efficiently generate animals with multiple biallelic mutations that can be transmitted to the next generation with high efficiency [18,28-32].”
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, background): “In vertebrates, coinjection of in vitro transcribed Cas9 mRNA and sgRNA into single-cell embryos can produce multi-gene, biallelic mutant animals with high efficiency; the multiple mutations can also be efficiently transmitted to the next generation [11–16].”