The effect of dietary inulin on aerobic bacteria associated with hindgut of Arctic charr (Salvelinus alpinus L.)

By E. Ringø, S. Sperstad, R. Myklebust, Terry M Mayhew, R. E. Olsen. 2006

155 citations recorded by the source corpus.

The primary aim of the present study was to evaluate the population level of adherent (autochthonous) aerobic and facultative anaerobic bacteria in the hindgut of healthy Arctic charr (Salvelinus alpinus L.) fed dextrin or inulin. This was assessed by the dilution plate technique, and visualized using both transmission and scanning electron microscopy. A population level of 4.8 × 105 adherent bacteria per gram wet mass was found in the hindgut of fish fed a casein-based diet supplemented with 15% dextrin. However, substituting dextrin with 15% inulin reduced the bacterial population level in the hindgut (3.56 × 104). A total of 217 bacterial isolates were identified by key phenotypical and biochemical characteristics. In addition, 22 strains were also identified by partial sequencing of the 16S rRNA gene. The composition of bacteria colonizing the hindgut of Arctic charr fed dextrin was dominated by the genera Staphylococcus, Pseudomonas, Micrococcus, Psychrobacter glacincola and Streptococcus. However, bacteria colonizing the hindgut of fish fed inulin were dominated by Gram-positive bacteria of the genera Staphylococcus, Streptococcus, Carnobacterium and Bacillus. While Carnobacterium divergens-like strains were isolated from charr fed dextrin, Carnobacterium maltaromicus-like strains were isolated from the hindgut of fish fed inulin. Electron microscopical analysis of hindgut regions confirmed traditional culture-based microbial analysis as fewer bacterial cells were observed between microvilli and associated with the surfaces of enterocytes of fish fed inulin rather than dextrin.

Subfields: Food Science, Immunology, Nutrition and Dietetics

View Paper: https://www.semanticscholar.org/paper/bb865377f8b325b65330dd300fb6b6d6d7d23dd8

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Food Science

1. E. Ringø
2. D. Merrifield
3. Zhigang Zhou
4. S. Davies
5. R. L. Olsen

In Immunology

1. E. Ringø
2. D. Merrifield
3. S. Davies
4. R. L. Olsen
5. Zhigang Zhou
6. Å. Krogdahl
7. S. Sperstad
8. A. Dimitroglou

In Nutrition and Dietetics

1. E. Ringø
2. R. L. Olsen
3. R. Myklebust

Citations in the corpus

• Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story? (2016, background): “More recently, Ringø et al. (2006a) showed that substituting 15% dextrin with 15% inulin resulted in a decrease in total viable counts and alterations of the adherent hindgut microbiota of Arctic charr.”
• Characterisation of the microbiota associated with intestine of Atlantic cod (Gadus morhua L.): The effect of fish meal, standard soybean meal and a bioprocessed soybean meal (2006, methodology): “Previous bacteriological investigations of the digestive tract of Arctic charr (Ringø et al., 2006) and Atlantic salmon (Bakke-McKellep et al.”