The GABAergic anterior paired lateral neuron suppresses and is suppressed by olfactory learning

By Xu Liu, Ronald L. Davis. 2008

244 citations recorded by the source corpus.

GABAergic neurotransmitter systems are important for many cognitive processes, including learning and memory. We identified a single neuron in each hemisphere of the Drosophila brain, the anterior paired lateral (APL) neuron, as a GABAergic neuron that broadly innervated the mushroom bodies. Reducing GABA synthesis in the APL neuron enhanced olfactory learning, suggesting that the APL neuron suppressed learning by releasing the inhibitory neurotransmitter GABA. Functional optical-imaging experiments revealed that the APL neuron responded to both odor and electric-shock stimuli that was presented to the fly with increases of intracellular calcium and released neurotransmitter. Notably, a memory trace formed in the APL neuron by pairing odor with electric shock. This trace was detected as a reduced calcium response in the APL neuron after conditioning specifically to the trained odor. These results demonstrate a mutual suppression between the GABAergic APL neuron and olfactory learning, and emphasize the functional neuroplasticity of the GABAergic system as a result of learning.

Subfields: Cellular and Molecular Neuroscience, Genetics, Insect Science

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Citations in the corpus

Re-evaluating circuit mechanisms underlying pattern separation (2019, neutral): “Global feedback inhibition is provided by a single GABAergic neuron, called the anterior paired lateral (APL) neuron in Drosophila, which is both presynaptic and postsynaptic to virtually all Kenyon cells (Leitch and Laurent, 1996; Liu and Davis, 2009; Papadopoulou et al., 2011), although recent evi”
Modelling learning and memory in Drosophila to understand Intellectual Disabilities. (2020, unknown): “Furthermore, GABAergic inputs to the MB, such as the anterior paired lateral neurons and the dorsal paired medial neurons, are implicated in behavioral flexibility, learning and memory consolidation (Lin et al., 2014a; Liu and Davis, 2009; Pitman et al., 2011; Ren et al., 2012; Wu et al., 2012).”
A connectome of a learning and memory center in the adult Drosophila brain (2017, background): “Second, overall KC excitability is regulated by feedback inhibition from a GABAergic neuron, MB-APL, that arborizes throughout the MB (Papadopoulou et al., 2011; Lin et al., 2014a; Tanaka et al., 2008; Liu and Davis, 2009).”
Are mushroom bodies cerebellum-like structures? (2011, background): “observed in several insect species (Gronenberg, 1987; Homberg et al., 1987; Leitch and Laurent, 1996; Bicker, 1999; Grunewald, 1999; Liu and Davis, 2009).”
A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom body (2011, unknown): “The ChaGAL80 strain was as described in [11] and was provided by Ronald Davis (Scripps Florida); GH146QF, QUAS mCD8::GFP, and uas mCD8::mCherry were as described in [29, 33].”
Development‐based compartmentalization of the Drosophila central brain (2010, background): “It is now possible to visualize neuronal activity in live brains (Wang et al., 2003, 2008; Marella et al., 2006; Liu and Davis, 2008).”
Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila (2011, background): “In Drosophila , the GABAergic APL neuron that projects onto the mushroom body shows a reduced response to the trained odo”
The Drosophila Mushroom Body: From Architecture to Algorithm in a Learning Circuit. (2020, background): “Another important factor is of course DAN activity, which is often very different across compartments (see, for example, Berry et al. 2012, Mao & Davis 2009, Yamagata et al. 2016).”
Optimal degrees of synaptic connectivity (2017, unknown): “In theDrosophilamushroombody, asingleGABAergic interneuron on each side of the brain inhibits the Kenyon-cell population globally (Liu and Davis, 2009; Figure 1B).”
Dopamine activity in projection neurons regulates short‐lasting olfactory approach memory in Drosophila (2022, background): “DANs in the protocerebral posterior lateral 1 (PPL1) cluster (Mao & Davis, 2009) project axons projecting to the vertical lobes of the MBs and are essential for olfactory aversive memory formation, since suppressing dopamine (DA) release from these neurons attenuates olfactory learning (Aso et al.,…”
Origins of Cell-Type-Specific Olfactory Processing in the Drosophila Mushroom Body Circuit. (2017, unknown): “KCs are also under a recurrent, inhibitory control of GABAergic anterior paired lateral (APL) neurons (Figure 1A; Lin et al., 2014; Liu and Davis, 2009; Tanaka et al., 2008).”