Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate
By Sayda M. Elbashir, Javier Martínez, Agnieszka Patkaniowska, Winfried Lendeckel, Thomas Tuschl. EMBO Journal, 2001
1,564 citations recorded by the source corpus.
Subfields: Biomaterials, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/b606205a102ebc3b1a610893b97d258a85084910
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In Biomaterials
In Molecular Biology
- David P. Bartel
- Phillip D. Zamore
- Gregory J. Hannon
- John J. Rossi
- Thomas Tuschl
- T. Rana
- Yukihide Tomari
- Scott M. Hammond
Citations in the corpus
- Illuminating the silence: understanding the structure and function of small RNAs (2007, background): “75–77 ) to catalyse the cleavage of target mRNA at a site that is ∼10 nt from the 5′ end of the siRN”
- Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain (2004, background): “siRNA is a 19–23-base-paired (bp) duplex with 2-nucleotide (nt) 3′ overhangs at both ends, containing 5′-phosphates and 3′-hydroxyl”
- Control of translation and mRNA degradation by miRNAs and siRNAs. (2006, background): “Third, for efficient endonuclease cleavage, base-pairing is needed at the site of cleavage, between bases 10 and 11 (Elbashir et al. 2001; Haley and Zamore 2004; Martinez and Tuschl 2004).”
- Gene silencing in mammals by small interfering RNAs (2002, background): “, particularly if the mismatches occur near the end of the duple”
- The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. (2002, background): “According to the current mechanistic model, dsRNAs initiate RNAi following their conversion into small, 21– 24-nucleotide (nt) short interfering RNAs (siRNAs; Hamilton and Baulcombe 1999; Zamore et al. 2000; Bernstein et al. 2001a; Elbashir et al. 2001).”
- A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans (2004, background): “The 3′ overhangs of siRNAs are required for efficient siRNA-mediated mRNA degradatio”
- MicroRNAS and their regulatory roles in plants. (2006, background): “Sequencing the resulting PCR product reveals the precise position of target cleavage, expected to be between nucleotides that pair to positions 10 and 11 of the miRNA (38).”
- Perspective: machines for RNAi. (2005, background): “Unlike RNase H, each cleavage-competent RISC can break only one phosphodiester bond (the “scissile phosphate”) in its RNA target (Elbashir et al. 2001a,b).”