DNA demethylation in the human FOXP3 locus discriminates regulatory T cells from activated FOXP3+ conventional T cells

By U. Baron, S. Floess, Georg Wieczorek, Katrin Baumann, A. Grützkau, Jun Dong, A. Thiel, Tina J. Boeld, P. Hoffmann, M. Edinger and 4 more. European Journal of Immunology, 2007

719 citations recorded by the source corpus.

Subfields: Epidemiology, Immunology

View Paper: https://www.semanticscholar.org/paper/a8c9b52a99ae0a52de395007649c2207f470062e

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In Epidemiology

Shimon Sakaguchi

In Immunology

Citations in the corpus

Regulatory T Cells and Human Disease. (2020, background): “It is also of note that, despite the expression of common proteins such as CD25 and CTLA-4 by Tregs and activated Tconvs, the epigenomic changes, in particular the Treg-specific CpG hypomethylation pattern, enable clear differentiation between Tregs and activated Tconvs at the genomic level, for exa”
Functional delineation and differentiation dynamics of human CD4+ T cells expressing the FoxP3 transcription factor. (2009, background): “The notion is also supported by recent reports showing that activation-induced FoxP3+CD4+ T cells have FOXP3 DNA significantly less demethylated than bona fide Treg cells (Baron et al., 2007; Janson et al., 2008).”
Plasticity of CD4+ T cell lineage differentiation. (2009, background): “Besides the promoter and an intronic enhancer, a highly conserved CpGrich element is present upstream of exon 1 in the Foxp3 locus and is referred to as the Treg cell-specific demethylated region (TSDR) (Floess et al., 2007; Kim and Leonard, 2007; Baron et al., 2007; Nagar et al., 2008).”
Control of regulatory T cell lineage commitment and maintenance (2009, background): “In contrast, these elements remain methylated in iTreg cells generated in vitro that do not stably express Foxp3 (Figure 2; Baron et al., 2007; Floess et al., 2007; Polansky et al., 2008).”
Regulatory T Cells: Mechanisms of Differentiation and Function (2012, background): “Intriguingly, these elements remain heavily methylated in in vitro–generated iTreg cells, which do not stably express Foxp3 (65, 181, 182).”
Plasticity of Foxp3(+) T cells reflects promiscuous Foxp3 expression in conventional T cells but not reprogramming of regulatory T cells. (2012, background): “What then distinguishes the committed state of Treg cells from promiscuous Foxp3 expression? We found that these two alternative modes of Foxp3 expression were associated with differential methylation status of the TSDR, supporting the notion that a demethylated TSDR distinguishes Treg cells from ac”