Induced pluripotent stem cells generated without viral integration

By R. Eggenschwiler, T. Cantz. Hepatology, 2009

1,098 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/a598bc5bc9fffb2d08ca28a45769531c44559266

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Citations in the corpus

The tumorigenicity of human embryonic and induced pluripotent stem cells (2011, background): “Therefore, a considerable amount of effort has recently been put into producing virus-free and integration-free mouse and human iPSCs using non-integrating adenoviruse”
Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation (2009, methodology): “Furthermore, the system presented herein can be used for packaging of integrase-deficient lentiviral vectors to facilitate tracking of transient reprogramming factor expression and screening of clones devoid of integrated vectors (8, 9, 34).”
Functional consequences of developmentally regulated alternative splicing (2011, background): “It will be particularly interesting to determine whether, like transcriptio”
Induced pluripotency: history, mechanisms, and applications. (2010, methodology): “…the inert drug doxycycline, diminishes the risk of continued transgene expression and allows for the selection of fully reprogrammed iPSCs, since cells that depend on exogenous factor expression readily stop proliferating upon doxycycline withdrawal (Brambrink et al. 2008; Stadtfeld et al. 2008b).”
Ethical and Safety Issues of Stem Cell-Based Therapy (2018, methodology): “Several types of non-integrating methods have been developed [use of non-integrating adenoviral vectors, repeated transfection of plasmids, Cre–loxP– mediated recombination, PiggyBac-transposition] [37-41].”
Parkinson’s Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors (2009, background): “So far, two different strategies have been pursued to generate genetically nonmodified iPSCs: (1) Reprogramming of mouse somatic cells has been achieved through transient expression of the reprogramming factors using either adenoviral infection or transient transfection (Okita et al., 2008; Stadtfel”
Oct4-induced pluripotency in adult neural stem cells. (2009, positive): “…(Oct4, Sox2, Klf4, and c-Myc) from mouse and human somatic cells (Lowry et al., 2008; Maherali et al., 2007; Meissner et al., 2007; Okita et al., 2007, 2008; Park et al., 2008; Stadtfeld et al., 2008; Takahashi et al., 2007; Takahashi and Yamanaka, 2006; Wernig et al., 2007; Yu et al., 2007).”
Pluripotency: Toward a gold standard for human ES and iPS cells (2009, background): “…of genomic integration can be overcome (Table 4) (Okita et al., 2007; JOURNAL OF CELLULAR PHYSIOLOGY Huangfu et al., 2008; Lowry and Plath, 2008; Stadtfeld et al., 2008). iPS technology has opened the door to a future of directed differentiation using a specific combination of multiple factors…”
Somatic cell reprogramming and transitions between pluripotent states: facts, hypotheses, unresolved issues (2010, neutral): “Furthermore, the generation of genetically unmodified iPSCs argues that insertional mutagenesis is not an essential step in the process (Okita et al., 2008; Stadtfeld et al., 2008).”
Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors (2011, methodology): “Toward this end, several techniques have been used for obtaining integration and/or transgene-free iPSCs, including the use of plasmids (3, 4), the Cre/ loxP system (5, 6), adenoviruses (7), piggyBac (8, 9), minicircle vector (10), and proteins (11, 12).”