Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria

By Elizabeth A. Ottesen, J. Hong, Stephen R. Quake, Jared R. Leadbetter. Science, 2006

687 citations recorded by the source corpus.

Subfields: Ecology, Evolution, Behavior and Systematics, Genetics

View Paper: https://www.semanticscholar.org/paper/a0ebc961a32b020a0403b7ea6253aa026855eea5

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology, Evolution, Behavior and Systematics

1. A. Brune
2. Moriya Ohkuma
3. C. Nalepa
4. N. Moran
5. Philipp Engel
6. H. Feldhaar

In Genetics

1. A. Brune
2. Moriya Ohkuma
3. C. Nalepa
4. N. Moran
5. Philipp Engel
6. H. Feldhaar

Citations in the corpus

• Microfabrication meets microbiology (2007, background): “A recent paper by Ottesen and co-workers elegantly demonstrated the use of microfluidic PCR to amplify and analyse genes isolated from single cells of bacteria harvested from the midgut of termite”
• Digital microfluidics: is a true lab-on-a-chip possible? (2007, background): “While numerous studies of on-chip PCR have been reported on continuous-flow microfluidic chips (Lagally et al. 2000; Schneegass et al. 2001; Cady et al. 2004; Ottesen et al. 2006), much less work has been reported on electrowetting or electrostatic-based PCR devices (Tokoro et al.”
• Role of the Termite Gut Microbiota in Symbiotic Digestion (2010, background): “Microfluidic digital PCR of individual gut bacteria confirmed that all FTHFS genes among the numerically predominant bacteria in the gut of Zootermopsis nevadensis belong to the uncultivated Treponema species typical of termite guts, but also indicated that not all treponemes in the community carry ”
• dPCR: A Technology Review (2018, neutral): “(Figure adapted from [65] with permission.”
• Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends (2007, background): “This affords an absolute readout of DNA copy number with single-molecule resolution (93), allowing for transcription factor profiling in individual hematopoietic progenitors (93) and multigene analysis of individual environmental bacteria (92).”
• Advances in Microfluidic PCR for Point-of-Care Infectious Disease Diagnostics (2011, methodology): “Detection of micro-organisms in pL scale chamber volumes with chip configurations of up to 1176 parallel reaction chambers have been reported (Marcus et al. , 2006b, Ottesen et al. , 2006, Pal et al. , 2005).”