Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends
By Chunsun Zhang, Da Xing. Nucleic Acids Research, 2007
411 citations recorded by the source corpus.
The possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. Devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply ‘Lab on a chip’ systems, have witnessed steady advances over the last several years. Here, we summarize recent research on chip substrates, surface treatments, PCR reaction volume and speed, architecture, approaches to eliminating cross-contamination and control and measurement of temperature and liquid flow. We also discuss product-detection methods, integration of functional components, biological samples used in PCR chips, potential applications and other practical issues related to implementation of lab-on-a-chip technologies.
Subfields: Biomedical Engineering
View Paper: https://www.semanticscholar.org/paper/b07181f9086788a6a4f7de90129118352320e993
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In Biomedical Engineering
Citations in the corpus
- Advances in Microfluidic PCR for Point-of-Care Infectious Disease Diagnostics (2011, background): “Many contact type heaters such as thin films, metal heating blocks, and Peltier units are commercially available at low cost and are thus widely adopted in on-chip PCR systems, while the temperature cycling speed can be limited by the thermal loss due to external coupling (Zhang and Xing, 2007).”
- Point-of-care diagnostics for global health. (2008, methodology): “Although the PCR platforms are ruggedized and adapted for field operation, they were inadequate in their ability to concentrate and purify nucleic acid at low concentration in large volumes of water.”