Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.

By Friedrich Fauser, Simon Schiml, Holger Puchta. The Plant Journal, 2014

716 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/9c6ab5a39d49ddb19860ce4e494ad41e709ae325

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In Molecular Biology

1. Jian‐Kang Zhu
2. C. Schwartz
3. R. Fischer
4. L. Bortesi
5. Joshua K. Young
6. S. Svitashev
7. Yong‐Bi Fu

In Plant Science

1. Jian‐Kang Zhu
2. R. Fischer
3. L. Bortesi
4. M. Ashraf
5. Kazuko Yamaguchi-Shinozaki
6. Kazuo Shinozaki
7. Viswanathan Chinnusamy
8. Ramanjulu Sunkar

Citations in the corpus

• Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, background): “However, transgenic lines expressing CRISPR/Cas9 have mainly been mosaic in the first generation (T1), indicating that CRISPR/Cas9-induced mutations in Arabidopsis occurred after the first embryonic cell division [20, 22, 25, 26, 29, 35].”
• A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. (2015, background): “…current plant CRISPR/Cas9 vector systems can only target one or few genomic sites (Jiang et al., 2013a, 2013b; Li et al., 2013; Mao et al., 2013; Shan et al., 2013a, 2013b; Xie and Yang, 2013; M Fauser et al., 2014; Feng et al., 2014; Xing et al., 2014; Zhang et al., 2014; Zhou et al., 2014).”
• The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “This strategy can yield precise genome modifications with efficiencies comparable to the unmodified enzyme, butwith 50- to 1500-fold greater specificity in human and mouse cells (Cho et al., 2014; Duda et al., 2014;Mali et al., 2013; Ran et al., 2013; Shen et al., 2014) and Arabidopsis (Fauser et al”
• Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA[OPEN] (2015, background): “…various sites may yield different results, incorporation of the Cas9 D10A nickase (Gasiunas et al., 2012) may further improve gene editing options for Cas9, because the nickase version has been successfully applied to improve specificity (Mali et al., 2013; Cho et al., 2014; Fauser et al., 2014).”
• Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, background): “…in tobacco, maize, Arabidopsis, tomato, rice, barley, flax, moss soybean and wheat (Shukla et al., 2009; Townsend et al., 2009; Zhang et al., 2013; Fauser et al., 2014; Budhagatapalli et al., 2015; Cermak et al., 2015; Li et al., 2015; Svitashev et al., 2015; Endo et al., 2016b; Li et al., 2016a;…”
• A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, methodology): “Agrobacterium-mediated transformation is a routine method used to generate transgenic plants, and a few binary vectors have been developed to deliver the CRISPR/Cas9 system into plant genomes via this method [15,20,23,24,33-40].”