In vivo two-photon calcium imaging of neuronal networks

By C. Stosiek, O. Garaschuk, K. Holthoff, Arthur Konnerth. Proceedings of the National Academy of Sciences of the United States of America, 2003

1,437 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/960a048a7b1f7d394bb66a484ea3ec8777422437

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Citations in the corpus

Dense connectomic reconstruction in layer 4 of the somatosensory cortex (2018, background): “Synaptic input variability Functional recordings of cortical neurons in-vivo show a remarkable variability of responses between neurons but also between stimulus exposures for a given neuron (Brecht and Sakmann, 2002; Kerr et al., 2007; Kerr et al., 2005; Ohki et al., 2005; Stosiek et al., 2003).”
Genetic dissection of neural circuits. (2008, positive): “To imagepopulationsof neurons, neural tissue is typically bulkloaded with membrane permeable [Ca2+] indicators (Yuste et al., 1992; Stosiek et al., 2003) or dextran-conjugated indicators (O’Donovan et al., 1993; O’Malley et al., 1996; Nagayama et al., 2007).”
Broadly tuned response properties of diverse inhibitory neuron subtypes in mouse visual cortex (2010, neutral): “A solution of 2 mM fluorescent dextran (10,000 MW Alexa-488 or Texas Red; Invitrogen) in ACSF was then pressure injected (5 to 20 pulses, <1 s each) through the 5 mm tip of a patch pipette positioned at the imaging site (Figure 2A).”
Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex (2005, methodology): “We adapted a recent protocol for bulk loading in vivo”
A disinhibitory microcircuit initiates critical period plasticity in visual cortex (2013, methodology): “Neurons in layer 2/3 of primary visual cortex were bulk labeled with the calcium indicator Oregon Green BAPTA-1, and changes in fluorescence intensity were imaged as described in references (38,39).”
Multi-electrode array technologies for neuroscience and cardiology. (2013, methodology): “These included imaging of membrane potentials by the use of voltage-sensitive dyes 23–27 , imaging of neuronal activity by monitoring the changes in the free intracellular calcium concentration 28–30 and the monitoring of intrinsic signals 31 .”
How advances in neural recording affect data analysis (2011, methodology): “Advances in dyes and scanning methods, as well as statistical methods for extracting spikes from fluorescence signals promise to make this approach feasible for thousands of neurons as wel”
Dendritic spines and distributed circuits. (2011, positive): “With two-photon calcium imaging, one can measure, for the first time, the functional properties of larger territories of cortex, while main- taining single-cell resolution (Ohki et al., 2005; Stosiek et al., 2003).”
Parvalbumin-expressing interneurons linearly control olfactory bulb output (2013, positive): “We next examined the odor-response properties of PV cells using in vivo two-photon calcium imaging in awake, head-fixed mice (Figure 3A) (Kato et al., 2012; Komiyama et al., 2010; Stosiek et al., 2003).”
The functional organization of the barrel cortex. (2007, methodology): “Neurons in layer 2/3 responding to whisker stimulation were already imaged in the first pioneering paper developing this technique for in vivo calcium imaging of network activity (Stosiek et al., 2003; Figure 3B).”