Cre recombinase: The universal reagent for genome tailoring

By A. Nagy. Genesis, 2000

1,377 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/8aa115e82c5b7e6de8a01cf16937bca2d1273af9

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Citations in the corpus

A highly efficient recombineering-based method for generating conditional knockout mutations. (2003, background): “This problem can be overcome by making conditional knockout mice (cko mice), which allows a gene to be inactivated in a tissue- or temporal-specific fashion (Nagy 2000).”
Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation/inactivation in the mouse. (2002, background): “The use of the P1 phage-derived integrase Cre to catalyze recombination between its loxP target sites has revolutionized the analysis of gene function in the mouse (Nagy, 2000; Rossant and McMahon, 1999; Rossant and Nagy, 1995).”
Split-Cre Complementation Indicates Coincident Activity of Different Genes In Vivo (2009, methodology): “The Cre/LoxP system [1] has been extensively used for cell fate”
A global double‐fluorescent Cre reporter mouse (2007, methodology): “…of Biological Sciences, Stanford University, Stanford, California 94305 3School of Medicine, Stanford University, Stanford, California 94305 Received 4 March 2007; Revised 8 July 2007; Accepted 12 July 2007 Summary: The Cre/loxP system has been used extensively for conditional mutagenesis in mice.”
Expression of Cre recombinase in the developing mouse limb bud driven by a Prxl enhancer (2002, background): “The paired-related homeobox gene-1 (Prx1, formerly called MHox1) is expressed in the early limb bud mesenchyme in both mouse (Cserjesi et al., 1992) and chick (Kuratani et al., 1994; Nohno et al., 1993) embryos.”
Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon cre‐mediated excision (2000, methodology): “To detect excision of floxed bgeo and expression of EGFP, germline-transmitting chimeric males and hemizygous F1 offspring were crossed with mice expressing pCX-NLS-Cre (Nagy, 2000).”
The zinc-finger transcription factor Klf4 is required for terminal differentiation of goblet cells in the colon. (2002, methodology): “Concerned that the loss of Klf4 might produce early lethality, we employed the Cre/loxP system to generate both Klf4-null mice and Klf4floxed mice, which could be used for conditional knockout experiments (Nagy, 2000).”
Genetic dissection of neural circuits. (2008, positive): “Because the same strategy has been used for Cre/loxP-mediated conditional knockouts, many transgenic mice expressing the Cre recombinase with different spatial and temporal patterns have been generated (reviewed in Nagy, 2000; Garcia-Otin and Guillou, 2006).”
A designer AAV variant permits efficient retrograde access to projection neurons (2016, background): “Even a low concentration of the Cre enzyme is sufficient to turn on the expression of such a Credependent cassette (Nagy, 2000), and the stringent nuclear localization of the histone-fused reporter affords unambiguous identification of infected cells, without confounding neuropil signal.”
Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis. (2010, methodology): “As a positive control, we used a Z/Red transgenic mouse line with deletion of the floxed stop in the germ line (hereafter referred to as NLSCre-Z/Red).(45,46) NLSCre-Z/ Red mice harbor the same transgene insertion site as the Z/Red mice, ruling out epigenetic differences in transgene expression betw”
Biology and Diseases of Mice (2015, neutral): “This approach can include insertion of reporter genes and selectable markers, and can be under the control of inducible gene expression systems (http:// www.eucomm.org/docs/protocols/mouse_protocol_1_ Sanger) (Nagy, 2000) .”