MicroRNA Expression in Zebrafish Embryonic Development

By Erno Wienholds, Wigard P. Kloosterman, E. Miska, Ezequiel Alvarez-Saavedra, Eugene Berezikov, Ewart de Bruijn, H. Horvitz, Sakari Kauppinen, Ronald H. A. Plasterk. Science, 2005

1,600 citations recorded by the source corpus.

MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid–modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, which suggests that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/8743b39bb43eaa89dc9c5481e05bb00ad8c566d9

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Citations in the corpus

Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. (2010, background): “Perhaps the best known example of a hairpin with two broadly conserved isoforms is mir-9, for which conserved miRNAs from both arms of the hairpin are readily detected by using in situ hybridization in both zebrafish and marine annelids (Wienholds et al. 2005; Christodoulou et al. 2010).”
Plant microRNA: a small regulatory molecule with big impact. (2006, background): “In animals, miRNAs have proven to be involved in many functional processes such as development (Wienholds et al., 2005; Giraldez et al., 2005), stress response (Ambros, 2003), and carcinogenesis (Iorio et al.”
Animal MicroRNAs confer robustness to gene expression and have a significant impact on 3'UTR evolution. (2005, positive): “We were notabletovisualize expressionofmiR-9bandc.Itispossible that they are expressed in proliferating neuronal precursor cells as in vertebrates ( Wienholds et al., 2005 ), where they might suppress premature differentiation, consistent with the miR-9 target spectrum.”
The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation (2006, result): “These data are consistent with findings in zebrafish showing that most miRNAs are expressed relatively late during embryogenesi”
microRNA modulation of circadian-clock period and entrainment. (2007, neutral): “Zebrafish in situ hybridization has shown that miR-219 is enriched in the brain (Wienholds et al., 2005).”
The evolution of cell types in animals: emerging principles from molecular studies (2008, background): “, which could be refined post-transcriptionally by the action of specific microRNA”
miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting. (2006, background): “…found that miRNAs are expressed not early in development but rather after tissue-fate specification, and this study concluded that miRNAs are likely to be more important for later differentiation and maintenance of tissue identity (Wienholds et al., 2005). miR-122 is one such tissue-specific miRNA.”
The evolution of gene regulation by transcription factors and microRNAs (2007, background): “Second, although miRNAs have not been detected in early zebrafish and medaka embryo”
Development of microRNA therapeutics is coming of age (2014, neutral): “In this section, we review selected examples, in which pharmacological modulation of miRNA activity has demonstrated a therapeutic benefit for the treatment of cancer, heart failure, atherosclerosis and HCV infection, respectively.”
Museum of spatial transcriptomics (2020, background): “(WM)ISH was the technology of choice in the late 1990s and the 2000s before the rise of highly multiplexed, high resolution, and more quantitative technologies, and has been used to create gene expression atlases in embryos of several species such as Drosophila melanogaster [25], Mus musculus, and G”