Mammalian Sir2 homolog SIRT7 is an activator of RNA polymerase I transcription.

By E. Ford, R. Voit, G. Liszt, C. Magin, I. Grummt, L. Guarente. Genes & Development, 2006

635 citations recorded by the source corpus.

We investigated the role of SIRT7, one of the seven members of the mammalian sirtuin family. We show that SIRT7 is a widely expressed nucleolar protein that is associated with active rRNA genes (rDNA), where it interacts with RNA polymerase I (Pol I) as well as with histones. Overexpression of SIRT7 increases Pol I-mediated transcription, whereas knockdown of SIRT7 or inhibition of the catalytic activity results in decreased association of Pol I with rDNA and a reduction of Pol I transcription. Depletion of SIRT7 stops cell proliferation and triggers apoptosis. Our findings suggest that SIRT7 is a positive regulator of Pol I transcription and is required for cell viability in mammals.

Subfields: Geriatrics and Gerontology, Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/8502f938e7e5801ba290df48db84871ef42a1eff

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Geriatrics and Gerontology

1. Johan Auwerx
2. E. Verdin
3. M. Haigis
4. L. Guarente
5. C. Cantó
6. R. Houtkooper
7. R. Mostoslavsky
8. F. Alt

In Molecular Biology

1. L. Guarente
2. Johan Auwerx
3. E. Verdin
4. R. Houtkooper
5. M. Haigis
6. R. Mostoslavsky
7. C. Cantó
8. G. Blander

In Physiology

1. Johan Auwerx
2. E. Verdin
3. C. Cantó
4. R. Houtkooper
5. M. Haigis
6. L. Guarente
7. R. Mostoslavsky
8. F. Alt

Citations in the corpus

• SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic beta cells. (2006, background): “SIRT7 is located in the nucleolus, where, analogous to the yeast Sir2p, it regulates transcription by RNA polymerase I (Ford et al., 2006).”
• The Role of Sirtuins in Antioxidant and Redox Signaling (2017, background): “SIRT7 overexpression has shown to increase RNA polymerase I (RNA Pol I)-mediated transcription, while knockdown or inhibition of SIRT7 decreases it (43).”
• NAD+/NADH and NADP+/NADPH in Cellular Functions and Cell Death: Regulation and Biological Consequences (2008, neutral): “A number of studies have suggested that sirtuins can also mediate gene expression through multiple pathways: First, both yeast Sir2 and mammalian SIRT1 can produce histone hypoacetylation and gene repression by promoting the formation of heterochromatin—-a tightly packed form of chromatin (200); sec”
• REACTIVE OXYGEN SPECIES, CELLULAR REDOX SYSTEMS AND APOPTOSIS (2010, neutral): “Beyond a reported involvement in maintaining genome integrity through activating base excision repair of oxidative damaged DNA or single-strand DNA breaks [94], the biological importance of Sirt6, a nucleolus-located deacetylase [95,96] is relatively unexplored.”
• Mammalian sirtuins--emerging roles in physiology, aging, and calorie restriction. (2006, background): “SIRT7 localizes to the nucleolus of human cells (Michishita et al. 2005; Ford et al. 2006).”
• Sirtuins: critical regulators at the crossroads between cancer and aging (2007, background): “SIRT7 regulation of rRNA genes in proliferating tissues SIRT7 localizes to nucleoli, associates with condensed chromosomes during mitosis and is widely expressed in proliferating mouse tissues, such as liver, testes and spleen (Michishita et al., 2005; Ford et al., 2006).”
• Sirtuins as regulators of metabolism and healthspan (2012, background): “, and SIRT7 was reported to reside in the nucleolu”
• The secret life of NAD+: an old metabolite controlling new metabolic signaling pathways. (2010, background): “As a consequence, the depletion of SIRT7 stopped cell proliferation and triggered apoptosis (125).”