Identification and characterization of enhancers controlling the inflammatory gene expression program in macrophages.

By S. Ghisletti, I. Barozzi, F. Mietton, S. Polletti, Francesca De Santa, E. Venturini, L. Gregory, L. Lonie, A. Chew, Chia-Lin Wei and 2 more. Immunity, 2010

668 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/81d2af5996d38a360b457adcc81bfe6404131358

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In Molecular Biology

1. K. Zaret
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Citations in the corpus

• Transcriptional regulation of macrophage polarization: enabling diversity with identity (2011, background): “1 is associated with nearly all genomic enhancers marked by H3K4me1 (Ref”
• Characterization of phenotype markers and neuronotoxic potential of polarised primary microglia in vitro (2013, methodology): “Analyses were performed with Graphpad 5.0 software (San Diego, CA, USA), and p 6 0.05 was accepted as statistically significant.”
• Latent enhancers activated by stimulation in differentiated cells. (2013, neutral): “…state reflects the fact that these enhancers are ignored because of a combination of two main factors: nucleosome occlusion and a comparatively low affinity for Pu.1, which would otherwise be able to invade nucleosomal DNA and induce enhancer formation (Ghisletti et al., 2010; Heinz et al., 2010).”
• PEDLA: predicting enhancers with a deep learning-based algorithmic framework (2016, methodology): “These approaches involve mapping the binding sites of enhancer indicative transcription factors (TFs) (26-29) or cofactors (30-32) by ChIP-Seq, identifying open chromatin with techniques such as DNase-Seq (33-35), and interrogating covalent histone modifications by ChIP-Seq (7,12,36,37).”
• Tissue-Resident Macrophage Enhancer Landscapes Are Shaped by the Local Microenvironment (2014, background): “enhancers, on the other hand, tend to be bound by specific sets of TFs that regulate their chromatin states (Ghisletti et al., 2010; Heinz et al., 2010; Lara-Astiaso et al., 2014; Winter and Amit, 2014).”
• Regulatory T Cells: Mechanisms of Differentiation and Function (2012, background): “1 is required for the maintenance of cell type– specific regulatory chromatin characteristics following macrophage and B cell differentiation (153, 154).”
• Diversity, Mechanisms and Significance of Macrophage Plasticity (2019, background): “Genome wide studies profiling transcriptional epigenetic modifications occurring in differentiated macrophages reveal profound dynamic changes in nucleosome positioning (43–45), histone modifications and DNA methylation patterns (41; 46; 47).”
• Functional and mechanistic diversity of distal transcription enhancers (2011, positive): “Enhancer predictions become more significant, however, when the histone methylation signature is combined with other indicators of enhancer activity in a specific cell type, such as transcription cofactor binding—most often, the acetyltransferase p300 (Visel et al., 2009a; Blow et al., 2010; Ghislet”
• Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities (2010, background): “…play a role in shaping the transcriptional response to inflammatory stimuli such as lipopolysaccharide (LPS), likely by generating cell type-specific regions of open chromatin that serve as beacons for the recruitment of transcriptional coactivators in response to stimuli (Ghisletti et al., 2010).”