Chromosomal silencing and localization are mediated by different domains of Xist RNA

By Anton Wutz, T. Rasmussen, R. Jaenisch. Nature Genetics, 2002

764 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/2348b2a3e1654a7730544aa2e5a6e65184e7141c

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Citations in the corpus

Mechanisms of Polycomb gene silencing: knowns and unknowns (2009, background): “XIST contains a 28 bp repeat element, called repA, which is required for silencing the inactive X chromosom”
Long non-coding RNA ANRIL is required for the PRC2 recruitment to and silencing of p15INK4B tumor suppressor gene (2010, background): “folds into two conserved stem-loop structures (Wutz et al., 2002; Zhao et al., 2008).”
Time-resolved structured illumination microscopy reveals key principles of Xist RNA spreading (2020, result): “Consistent with these observations, prior work reported that the A-repeat is critical for in cis localization of a truncated Xist RNA transgene (28).”
A decade of 3C technologies: insights into nuclear organization. (2012, background): “The noncoding RNA Xist drives X inactivation, but is not required to maintain gene silencing (Wutz et al. 2002).”
Non-coding RNAs as regulators of embryogenesis (2011, background): “Absence of Tsix expression on Xi enables expression of an internal transcription unit within the Xist transcript called repeat A ( RepA”
Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes. (2003, background): “A recent study demonstrated that the silencing function of Xist RNA maps to a short tandemly repeated element at the 5 end of the transcript (Wutz et al., 2002).”
Chromatin modification and epigenetic reprogramming in mammalian development (2002, background): “A recent study of full-length Xist cDNA and of various deleted forms of Xist identified that the 5′ region of Xist is required for long-range silencing on the inactive X, whereas several other regions of the Xist RNA are required for Xist to coat the X chromosom”
lincRNAs: Genomics, Evolution, and Mechanisms (2013, neutral): “Interestingly, the first exon of Xist, which contains most of the known functional repetitive elements (Beletskii et al., 2001; Wutz et al., 2002; Sarma et al., 2010), is characterized by low PhastCons scores, perhaps because some of these repeats contain short functional sequences interspersed…”
A cytoplasmic NF-κB interacting long noncoding RNA blocks IκB phosphorylation and suppresses breast cancer metastasis. (2015, background): “It has long been acknowledged that RNA is a malleable evolutionary substrate compared with protein, and mutation or deletion of the regions outside its functional domains may not interfere with its core functionality (Wutz et al., 2002).”
X-inactivation, imprinting, and long noncoding RNAs in health and disease. (2013, background): “Deleting the noncoding locus Xist results in loss of silencing capability in cis (Penny et al., 1996; Marahrens et al., 1997), and placing the Xic at an ectopic location results in counting, choosing, and silencing of the autosome (Lee et al., 1996; Migeon et al., 1999; Wutz et al., 2002).”
A novel role for Xist RNA in the formation of a repressive nuclear compartment into which genes are recruited when silenced. (2006, methodology): “For MeCP2, Pgk1, G6pdx, and Lamp2, gene locations relative to the Xist RNA domain were also found to differ significantly (p 0.05) between days 2 and 4 of differentiation and MEFs, using a 2 test (see Materials and Methods).”
RNA duplex map in living cells reveals higher order transcriptome structure (2016, background): “The key region for XIST-mediated epigenetic silencing, termed the A-repeat, is comprised of 7.5 or 8.5 near-identical copies of a sequence, and multiple structural models have been proposed (Fang et al., 2015; Maenner et al., 2010; Wutz et al., 2002).”