RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.

By Phillip D. Zamore, Thomas Tuschl, Phillip A. Sharp, David P. Bartel. Cell, 2000

2,853 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/73373a156cb24b578938096b18012b1f0ea1f574

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In Molecular Biology

Citations in the corpus

Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. (2002, background): “clear TAg staining were counted as positive (cytoplasmic staining mond et al., 2000; Zamore et al., 2000).”
Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. (2002, background): “The arrow indicates the 5Ј cleavage product; the 3Ј fragment is unlabeled and therefore invisible. tal Figure S2 available at http://www.cell.com/cgi/ PCR (Lipardi et al., 2001).”
Current prospects for RNA interference-based therapies (2011, background): “siRNAs generally show full complementarity to their target mRNA, and cleavage occurs 10-12 bases from the 5′ end of the guide strand binding site 3, 28 .”
miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. (2002, background): “In RNAi, small interfering RNAs (siRNAs) of 21–23 nt recognize homologous mRNAs and silence gene expression by degradation of the mRNA (Fire et al. 1998; Bass 2000; Zamore et al. 2000; Elbashir et al. 2001; Sharp 2001).”
Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. (2005, neutral): “In Drosophila embryo lysate lacking Ago2, siRNAs remain double stranded (Okamura et al., 2004) and stably bound to Dcr-2 and R2D2 (Tomari et al., 2004b) ,”
Functional siRNAs and miRNAs exhibit strand bias. (2003, neutral): “siRNAs have been shown to act as the functional inter- these data suggest that the thermodynamic properties of siRNA molecules play a central role in determining mediate in RNAi, specifically directing cleavage of complementary mRNA targets in a process that is commonly the molecule’s functionality ”
CRISPR interference (CRISPRi) for sequence-specific control of gene expression (2013, background): “Several targeted gene regulation techniques have been widely used in the past, such as RNA interference (RNAi) 27,28 or engineered DNA-binding proteins, including zinc-finger 29–31 or transcription activator–like effector (TALE) 32–34 proteins.”