Analysis of a Complete Library of Putative Drug Transporter Genes in Escherichia coli

By K. Nishino, A. Yamaguchi. Journal of Bacteriology, 2001

681 citations recorded by the source corpus.

ABSTRACT The complete sequencing of bacterial genomes has revealed a large number of drug transporter genes. In Escherichia coli, there are 37 open reading frames (ORFs) assumed to be drug transporter genes on the basis of sequence similarities, although the transport capabilities of most of them have not been established yet. We cloned all 37 putative drug transporter genes in E. coli and investigated their drug resistance phenotypes using anE. coli drug-sensitive mutant as a host. E. coli cells transformed with a plasmid carrying one of 20 ORFs, i.e., fsr, mdfA, yceE,yceL, bcr, emrKY,emrAB, emrD, yidY,yjiO, ydhE, acrAB, cusA(formerly ybdE), yegMNO,acrD, acrEF, yhiUV,emrE, ydgFE, and ybjYZ, exhibited increased resistance to some of the 26 representative antimicrobial agents and chemical compounds tested in this study. Of these 20 ORFs,cusA, yegMNO, ydgFE,yceE, yceL, yidY, andybjYZ are novel drug resistance genes. The fsr, bcr, yjiO, ydhE, acrD, and yhiUVgenes gave broader resistance spectra than previously reported.

Subfields: Ecology, Genetics, Molecular Medicine

View Paper: https://www.semanticscholar.org/paper/6e68abefdfaaf3035cd260bf6d7cf339d6742352

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. L. Piddock
2. Xian-Zhi Li
3. D. Nies
4. H. Nikaido
5. G. von Heijne

In Genetics

1. L. Piddock
2. Xian-Zhi Li
3. D. Nies
4. H. Nikaido
5. G. von Heijne

In Molecular Medicine

1. Xian-Zhi Li
2. H. Nikaido
3. L. Piddock
4. D. Nies
5. G. von Heijne
6. J. Pagés
7. Gerard D. Wright
8. T. Grossman

Citations in the corpus

• Clinically Relevant Chromosomally Encoded Multidrug Resistance Efflux Pumps in Bacteria (2006, background): “coli, acrD and the acrEF operon also encode efflux pumps (188, 236), and AcrD has been shown to efflux aminoglycosides (140, 188).”
• Membrane-protein topology (2006, background): “, whereas YdgE and YdgF need to be co-expressed to confer drug resistanc”
• Efflux-mediated antimicrobial resistance. (2005, neutral): “coli also accommodates QACs.(365,366) A Pseudomonas fluorescens isolate contaminating a batch solution of benzalkonium chloride and showing high-level resistance to multiple QACs that was compromised Review”
• Multidrug Resistance in Bacteria (2009, background): “coli can confer increased resistance to cationic dyes, chloramphenicol, and fluoroquinolones when overexpressed from plasmids in a mutant strain lacking the constitutive RND pump AcrB (60), although mdfA deletion produces no effect on drug susceptibility (61).”
• Efflux-Mediated Drug Resistance in Bacteria: an Update (2009, background): “However, LacY and GlpT permeases transport a relatively narrow range of structurally related substrates,([72,73]) while EmrD accommodates a range of hydrophobic agents including benzalkonium, carbonyl cyanidem-chlorophenylhydrazone and sodium dodecylsulfate.([1,75]) The EmrD structure demonstrates a”
• Efflux-mediated heavy metal resistance in prokaryotes. (2003, background): “The di¡erent substrate speci¢cities of all six HAE-RNDs have been characterized [110,111], but most data are available for the AcrAB-TolC system [98,112^115] that protects the bacterium against a variety of toxic substances especially during slow growth [116].”