Preliminary characterization of bacteriocins from Lactococcus lactis, Enterococcus faecium and Enterococcus mundtii strains isolated from turbot (Psetta maxima)
By C. Campos, O. Rodríguez, P. Calo-Mata, M. Prado, J. Barros-Velázquez. 2006
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The aim of this work was the characterization of new strains of lactic acid bacteria (LAB) from farmed fish and with potential application as biopreservatives against both Listeria monocytogenes and Staphylococcus aureus. Twenty-five strains of LAB isolated from the muscle of farmed turbot were investigated. Genetic identification of the bacteriocin-producing LAB strains was performed by means of a PCR method using novel BAL1/BAL2 16S ribosomal-RNA-targeted primers. Maximum bacteriocin production by Lactococcus lactis ssp. lactis USC-39, Enterococcus faecium USC-46 and Enterococcus mundtii USC-51 was detected in the stationary phase of growth. Both acidification and the production of hydrogen peroxide by LAB were ruled out as the source of the inhibition. In contrast, the antimicrobial activity of all three LAB strains was inactivated by the addition of proteinase K, thus confirming the proteinaceous nature of the inhibition. The activity against L. monocytogenes was maintained in the 3.5-5.5 or 3.5-6.5 pH range, depending on the LAB strain. Likewise, inhibition of S. aureus strains was observed in the 3.5-4.5 and in the 3.5-5.5 pH ranges, depending on the LAB strain and on the S. aureus strain tested. Bacteriocin activity was stable in all three strains after heating the cell-free extract for 60 min at 100 °C, or even for 15 min at 121 °C, in all the three LAB strains. The acidic and heat-resistant bacteriocins produced by the three LAB strains isolated from turbot, able to inhibit the growth of both L. monocytogenes and S. aureus may find application as biopreservatives in fermented and/or heated food products.
Subfields: Animal Science and Zoology, Food Science, Immunology
View Paper: https://www.semanticscholar.org/paper/6cd4acb23e78e84793c299835a433834db638f94
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Citations in the corpus
- Probiotics in aquaculture: a current assessment (2014, background): “Previous studies have shown that some endogenous lactic acid bacteria (LAB), such as those belonging to the genus Carnobacterium, Lactobacillus, Lactococcus and Enterococcus, are able to produce bacteriocins that can inhibit the growth of some pathogenic bacteria (Ringø & Gatesoupe 1998; Campos et a”
- Antimicrobial activity and partial characterization of bacteriocins produced by lactobacilli isolated from Sturgeon fish (2013, background): “In fish LAB are naturally present, and the bio-protective potential of some strains and/or their bacteriocin has been highlighted in the last years (Bucio, Hartemink, Schrama, Verreth, & Rombouts, 2006; Campos et al., 2006; Ghanbari, Rezaei, Jami, & Nazari, 2009; Ghanbari, Rezaei, Soltani, & Shahoss”
- Current Applications and Future Trends of Lactic Acid Bacteria and their Bacteriocins for the Biopreservation of Aquatic Food Products (2008, background): “In fresh turbot, the composition of the microflora is mainly Enterococcus (more than 90% of isolated bacteria from turbot muscle; Campos et al. 2006).”
- The ecology, epidemiology and virulence of Enterococcus. (2009, background): “Enterococcus species are known to produce a range of enterocins (Table 1) including enterocins A, B, I, L and P, which are active against Listeria species, Clostridum species and Staphylococcus aureus (Campos et al., 2006).”