Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells
By Y. Teng, E. Lavik, Xianlu Qu, K. Park, J. Ourednik, D. Zurakowski, Robert Langer, E. Snyder. Proceedings of the National Academy of Sciences of the United States of America, 2002
978 citations recorded by the source corpus.
To better direct repair following spinal cord injury (SCI), we designed an implant modeled after the intact spinal cord consisting of a multicomponent polymer scaffold seeded with neural stem cells. Implantation of the scaffold–neural stem cells unit into an adult rat hemisection model of SCI promoted long-term improvement in function (persistent for 1 year in some animals) relative to a lesion-control group. At 70 days postinjury, animals implanted with scaffold-plus-cells exhibited coordinated, weight-bearing hindlimb stepping. Histology and immunocytochemical analysis suggested that this recovery might be attributable partly to a reduction in tissue loss from secondary injury processes as well as in diminished glial scarring. Tract tracing demonstrated corticospinal tract fibers passing through the injury epicenter to the caudal cord, a phenomenon not present in untreated groups. Together with evidence of enhanced local GAP-43 expression not seen in controls, these findings suggest a possible regeneration component. These results may suggest a new approach to SCI and, more broadly, may serve as a prototype for multidisciplinary strategies against complex neurological problems.
Subfields: Cellular and Molecular Neuroscience, Pathology and Forensic Medicine, Surgery
View Paper: https://www.semanticscholar.org/paper/5d09ddcda83f35e308c8edbf03660631d8631c3c
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Citations in the corpus
- Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis (2003, background): “They support neurogenesis within restricted areas throughout adulthood, can undergo extensive in vitro expansion and, therefore, have been proposed as a renewable source of neural precursors for regenerative transplantation in various CNS disease”
- Secondary injury mechanisms in traumatic spinal cord injury: a nugget of this multiply cascade. (2011, background): “…(Papastefanaki et al. 2007), transplantation of olfactory unsheathing cells (Li et al. 2003), transplantation of embryonic stem/progenitor cells (Teng et al. 2002, Jablonska et al. 2010, Szymczak et al. 2010), transplantation of adult stem/progenitor cells (Koda et al. 2005, Karimi-Abdolrezaee…”
- Three-Dimensional Cell Culture Matrices: State of the Art (2008, methodology): “developed a PLGA-based 3Dmatrix with 2 distinct structures to treat spinal cord injuries.(155) The inner and outer regions of the 3D matrix were fabricated separately and then combined.”
- The Biology of Regeneration Failure and Success After Spinal Cord Injury. (2018, background): “The inflammatory post-SCI environment, for example, can be modulated by systemic, trophic factor producing stem cells (13) such as multipotential adult progenitor cell treatment (FIGURE 3E) to confer neuroprotection (89, 309, 385, 404) [possibly via their effects in the spleen (88)] and to promote n”
- Mesenchymal stem cells: clinical applications and biological characterization. (2004, background): “Stem cells with the ability to differentiate into neurons, astrocytes and oligodendrocytes have been isolated from rat spinal cord (Shihabuddin, Horner, Ray, & Gage, 2000), and implantation of neural stem cells in an adult rat model of spinal cord injury resulted in long-term functional improvement ”
- The therapeutic potential of neural stem cells (2006, background): “Embryonic, foetal and neonatal cerebellar NPCs injected into models of SCI do not differentiate into terminally differentiated neuronal cell”
- Self-Assembling Nanofibers Inhibit Glial Scar Formation and Promote Axon Elongation after Spinal Cord Injury (2008, background): “Numerous previous studies have used a variety of natural (e.g., collagen, agarose, and alginates) and synthetic (e.g., poly -hydroxy acids and polyvinylchloride) polymers for repair of the damaged spinal cord or brain (Teng et al., 2002; Stokols and Tuszynski, 2004, 2006; Jain et al., 2006).”
- Neural tissue engineering: strategies for repair and regeneration. (2003, neutral): “As part of a separate study from this same laboratory, a “two-phase” conduit was fabricated for spinal cord repair (159).”