Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes

By L. Álvarez-Erviti, Y. Seow, HaiFang Yin, Corinne A Betts, Samira Lakhal, M. Wood. Nature Biotechnology, 2011

4,482 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/56fe0f648bd5177d70b2f2678cf13db7b5494f1e

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Citations in the corpus

Naturally Occurring Exosome Vesicles as Potential Delivery Vehicle for Bioactive Compounds (2019, background): “Moreover, fetal calf serum exosome has proven to be a good vehicle for delivering short interfering (si)RNA into the mouse brain (Alvarez-Erviti et al., 2011).”
Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. (2014, background): “…receptor containing an amino acid sequence recognized by receptors specifically expressed on either neuronal or muscle cells were readily bound to a neuron or muscle cell line, respectively, in vitro (Alvarez-Erviti et al. 2011). www.annualreviews.org • Secreted Membrane Vesicles 277 A n n u .”
The biology, function, and biomedical applications of exosomes (2020, positive): “RVG (rabies virus glycoprotein)–modified dendritic cell–derived exosomes with therapeutic Bace1-targeting siRNA were intravenously administrated to mice and the results showed suppression of BACE1 expression in the brain, a potential target for the treatment of Alzheimer’s disease (142).”
Exosomes as new vesicular lipid transporters involved in cell-cell communication and various pathophysiologies. (2014, methodology): “This observation has been further strengthened by inactivation of the brain protease BACE by means of bio-engineered exosomes injected into the tails of mice [49].”
Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation (2012, neutral): “Recent studies have highlighted the role of EVs in intercellular communication [20–22], vaccine and drug delivery [23– 25], and suggested a potential role in gene vector therapy [26] and as disease biomarkers [27].”
Using exosomes, naturally-equipped nanocarriers, for drug delivery (2015, methodology): “As an example, targeting of exosomes to the brain was achieved by engineering the parental DCs to express lysosomal-associated membrane protein 2 (Lamp2b), fused to the neuron-specific peptide derived from rabies virus glycoprotein (RVG) [13].”
Exosomes in cancer development, metastasis, and immunity (2019, background): “This study demonstrated the feasibility of exosome-specific systemic delivery and, more importantly, shows that exosome can pass biological barriers, shedding light on the possibility of new RNAi-based therapy for brain tumors, brain metastases, and neurodegenerative diseases [155].”
Designer exosomes produced by implanted cells intracerebrally deliver therapeutic cargo for Parkinson’s disease treatment (2018, neutral): “The supernatant containing the designer exosomes was applied to target HEK-293T cells expressing CHRNA7 without further concentration of the exosomes, followed by a luminescence assay after translation from nluc mRNA delivered to the target cells.”
Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications (2020, neutral): “As the name implies, post-secretory drug loading refers to the direct addition of therapeutic agents to exosomes in a certain way, but exosome aggregation, membrane damage and low yield may exist 101,126,127 .”