Isolation and Characterization of Tumorigenic, Stem-like Neural Precursors from Human Glioblastoma

By R. Galli, Elena Binda, U. Orfanelli, B. Cipelletti, A. Gritti, Simona De Vitis, R. Fiocco, C. Foroni, F. DiMeco, Angelo Luigi Vescovi. Cancer Research, 2004

2,640 citations recorded by the source corpus.

Subfields: Developmental Neuroscience, Genetics, Oncology

View Paper: https://www.semanticscholar.org/paper/50d893ccd164f79517deeee00d4f2c3ab5e8b158

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Citations in the corpus

Highly tumorigenic lung cancer CD133+ cells display stem-like features and are spared by cisplatin treatment (2009, background): “Normal and neoplastic stem-like cells from neural and epithelial organs can be expanded as sphere-like aggregates in serum-free EGF–bFGF-supplemented medium (9, 15, 36) that favors the proliferation of undifferentiated cells.”
Hypoxia promotes expansion of the CD133-positive glioma stem cells through activation of HIF-1α (2009, background): “Gliomas, the most common primary brain tumor, are believed to be initiated and maintained by cancer stem cells (CSCs), a population of cells capable of extensive self-renewal, differentiation into multiple lineages and recapitulation of the original tumor in immunodeficient mice (Galli et al., 2004;”
A tumorigenic subpopulation with stem cell properties in melanomas. (2005, background): “CSCs from brain tumors could be isolated, because they show in vitro growth characteristics similar to those of neural stem cells, which proliferate as nonadherent cell aggregates termed spheres or spheroids (8, 9).”
Integrin alpha 6 regulates glioblastoma stem cells (2010, background): “…cells (CSCs), tumor-initiating cells, or tumor-propagating cells, has been found in many tumors (Reya et al., 2001) including brain cancers (Bao et al., 2006a, 2006b; Galli et al., 2004; Hemmati et al., 2003; Ignatova et al., 2002; Singh et al., 2003, 2004; Taylor et al., 2005; Yuan et al., 2004).”
Cancer stem cells: models and concepts. (2007, background): “succeeded in the isolation and serial propagation from human glioblastoma multiforme of “cancer neurospheres,” which are highly enriched in long-term selfrenewing, multi-lineage-differentiating, and tumor-initiating cells (60).”
Stem and progenitor-like cells contribute to the aggressive behavior of human epithelial ovarian cancer. (2005, background): “An understanding of the emergence and organization of solid cancers at the cellular level has only recently been addressed, and has resulted in the prospective isolation and characterization of cancer stem cells in breast (5) and brain (6, 7).”
Molecular subclasses of high-grade glioma predict prognosis, delineate a pattern of disease progression, and resemble stages in neurogenesis. (2006, background): “, 2004) and that human GBMs contain tumorigenic neural stemlike cells (Galli et al., 2004; Ignatova et al., 2002; Singh et al., 2004) indicate that neural stem and/or progenitor cells are a plausible origin for human gliomas and have given rise to speculation that more effective therapies will resul”
An integrated genomic analysis identifies clinically relevant subtypes of glioblastoma characterized by abnormalities in PDGFRA, IDH1, EGFR and NF1 (2010, background): “It is possible that a common cell of origin, such as the previously proposed neural stem cell (Galli et al., 2004), exists for all GBMs and that the classes presented here result from distinct differentiation paths.”
Phosphorylation of EZH2 activates STAT3 signaling via STAT3 methylation and promotes tumorigenicity of glioblastoma stem-like cells (2013, background): “…2 (FGF2) in culture media decreased expression of stem-cell-associated markers such as SOX2 and concurrently increased an astroglial marker expression (e.g., GFAP), consistent with a ‘‘differentiated’’ phenotype (Galli et al., 2004; Lee et al., 2006a; Piccirillo et al., 2006; Zheng et al., 2010).”
IDH1 mutation is sufficient to establish the glioma hypermethylator phenotype (2012, methodology): “Neurosphere assay IDH1(R123H)-expressing astrocytes and parental controls were grown in media permissive of neural stem cell growth as previously describe”
A perivascular niche for brain tumor stem cells. (2007, background): “Normal neural stem and precursor cells and brain CSCs express the major intermediate filament protein Nestin (Galli et al., 2004; Hemmati et al., 2003; Palmer et al., 2000; Singh et al., 2004; Taylor et al., 2005).”
Cancer stem cells in glioblastoma (2015, methodology): “Informed by techniques used to enrich and characterize NSPCs, several groups in parallel demonstrated that gliomas and other primary brain tumors contain self-renewing, tumorigenic cells (Ignatova et al. 2002; Hemmati et al. 2003; Singh et al. 2003, 2004; Galli et al. 2004).”
Rembrandt: Helping Personalized Medicine Become a Reality Through Integrative Translational Research (2009, background): “Suppose that one would have come across two publications on Glioma Tumor Stem Cells that mentioned the irregular expression of BMPR1B in such cells (9, 10).”