Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts

By C. Frezza, S. Cipolat, L. Scorrano. Nature Protocols, 2007

1,209 citations recorded by the source corpus.

Subfields: Clinical Biochemistry, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/50a7e66a527a1bf883b40f4c77be4f26a6893ee7

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Citations in the corpus

Mitochondrial fusion is required for mtDNA stability in skeletal muscle and tolerance of mtDNA mutations (2010, positive): “DNA Isolation To isolate mitochondrial DNA, cells or tissue were first homogenized and a mitochondrial fraction was isolated according to previously published protocols (Frezza et al., 2007).”
Mitochondrial Cristae Shape Determines Respiratory Chain Supercomplexes Assembly and Respiratory Efficiency (2013, methodology): “Mitochondria from liver of mice and from the indicated cell lines were isolated as in Frezza et al. (2007). Cytochrome c release, ascorbate/TMPD-driven respiration and cytochrome b5-dependent NADH oxidation were determined as described (Scorrano et al.”
The Metabolic Profile of Tumors Depends on both the Responsible Genetic Lesion and Tissue Type (2012, methodology): “Enzyme Assays A mitochondrial fraction was isolated from freshly excised normal livers or tumors as described previously (Frezza et al., 2007).”
Dimers of mitochondrial ATP synthase form the permeability transition pore (2013, methodology): “Immunoprecipitation (IP) of ATP synthase was performed from 0.5 mg bovine, mouse heart, or human osteo-sarcomaHQB17cellsmitochondriapreparedbystandardmethods(44,45)using anticomplex V monoclonal antibody covalently linked to protein G-Agarose beads (MS501 immunocapture kit; Abcam) as reported (24).”
Dephosphorylation by calcineurin regulates translocation of Drp1 to mitochondria (2008, neutral): “33.”
During autophagy mitochondria elongate, are spared from degradation and sustain cell viability (2011, methodology): “Cells plated in 500 cm 2 plates were treated after 48 h as indicated and mitochondria were isolated as described previousl”
Tumor-derived hydrogen sulfide, produced by cystathionine-β-synthase, stimulates bioenergetics, cell proliferation, and angiogenesis in colon cancer (2013, methodology): “HCT116 cells were subjected to subcellular fractionation by centrifugation (41).”
SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function (2012, methodology): “Mitochondrial Function and Citrate Synthase Activity Skeletal muscle mitochondria were isolated as described previously (Frezza et al., 2007), with minor modifications.”
Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells (2009, neutral): “Various protocols are available to obtain mitochondri”
Adaptation of hepatic mitochondrial function in humans with non-alcoholic fatty liver is lost in steatohepatitis. (2015, positive): “Mitochondria were isolated by differential centrifugation (Frezza et al., 2007).”
Miro1 Is a Calcium Sensor for Glutamate Receptor-Dependent Localization of Mitochondria at Synapses (2009, positive): “Mitochondria were then isolated as previously described (Frezza et al., 2007).”