Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells

By M. Wieckowski, C. Giorgi, Magdalena Lebiedzińska, J. Duszyński, P. Pinton. Nature Protocols, 2009

900 citations recorded by the source corpus.

Subfields: Clinical Biochemistry, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b8c7fcbc0bc4f1205e28560d8331a72f28bbc70b

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. M. Clatworthy
2. Ze Wang
3. N. Subramanian
4. Ronald N. Germain
5. K. Natarajan

Citations in the corpus

• Energy Metabolism in Human Pluripotent Stem Cells and Their Differentiated Counterparts (2011, methodology): “Mitochondrial extracts were isolated using mitochondria isolation kit (Sigma-Aldrich), as previously described by Wieckowski and colleagues [36].”
• The adapter MAVS promotes NLRP3 mitochondrial localization and inflammasome activation (2013, positive): “Mitochondria were isolated from cells using a Mitochondria Isolation Kit (Thermo Scientific) or as previously described (Wieckowski et al., 2009), lysed in 2% SDS, and boiled with 23 reducing sample buffer for SDS-PAGE.”
• The MBOAT7-TMC4 Variant rs641738 Increases Risk of Nonalcoholic Fatty Liver Disease in Individuals of European Descent (2016, positive): “In hepatoblastoma cells stably overexpressing MBOAT7 the recombinant protein was localized specifically in membrane fractions rich in phospholipids 34 i.e.: the endoplasmic reticulum and the mitochondria-associated membranes.”