Mitochondrial Cristae Shape Determines Respiratory Chain Supercomplexes Assembly and Respiratory Efficiency

By S. Cogliati, C. Frezza, M. Soriano, T. Varanita, Rubén Quintana-Cabrera, Mauro Corrado, S. Cipolat, Veronica Costa, A. Casarín, Lígia C. Gomes and 5 more. Cell, 2013

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Summary Respiratory chain complexes assemble into functional quaternary structures called supercomplexes (RCS) within the folds of the inner mitochondrial membrane, or cristae. Here, we investigate the relationship between respiratory function and mitochondrial ultrastructure and provide evidence that cristae shape determines the assembly and stability of RCS and hence mitochondrial respiratory efficiency. Genetic and apoptotic manipulations of cristae structure affect assembly and activity of RCS in vitro and in vivo, independently of changes to mitochondrial protein synthesis or apoptotic outer mitochondrial membrane permeabilization. We demonstrate that, accordingly, the efficiency of mitochondria-dependent cell growth depends on cristae shape. Thus, RCS assembly emerges as a link between membrane morphology and function.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/5ddab71028aa9c85f905f74dbdaf7e34d54c8a4a

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Citations in the corpus

Mitochondrial Cristae: Where Beauty Meets Functionality. (2016, background): “Recently, it was shown that cristae shape regulates [12_TD$DIFF]SCs stability and assembly, affecting cellular growth and lending a potential molecular explanation for the pathogenesis of DOA [11].”
Fatty acid trafficking in starved cells: regulation by lipid droplet lipolysis, autophagy and mitochondrial fusion dynamics (2015, methodology): “Mfn1KO cells were used as a primary model since, unlike Opa1KO and Mfn1/2KO MEFs (Chen et al., 2005; Cogliati et al., 2013; Frezza et al., 2006) (Figure S5A), their respiration levels under fed conditions (Figures 5E and 5F) and mitophagy levels under starvation conditions (Figure S5B) were…”
Metabolic regulation of mitochondrial dynamics (2016, background): “, 2015), and Opa1 maintains mitochondrial cristae structure and is critical for respiratory chain supercomplex assembly (Cogliati et al., 2013).”
Metabolic Instruction of Immunity (2017, neutral): “Supercomplexes facilitate efficient transfer of electrons and minimize proton leak during ATP production (Cogliati et al., 2013).”
Mitochondrial dynamics in health and disease: mechanisms and potential targets (2023, background): “In addition to controlling the IMM fusion, Opa1 also orchestrates cristae integrity, mtDNA maintenance, bioenergetics, as well as respiratory chain super complex assembly, so that Opa1 directly affects mitochondrial cytochrome release and oxidative respiration efficiency.(51,66) Early embryonic leth”
Multi-omics analysis identifies ATF4 as a key regulator of the mitochondrial stress response in mammals (2017, background): “Of note, mitochondrial stress also decreased the protein levels of OPA1 (Table S5), a mitochondrial inner membrane GTPase that participates in the inner membrane fusion and cristae maintenance (Frezza et al., 2006; Cogliati et al., 2013).”