Real‐Time Polymerase Chain Reaction

By J. Wilhelm, Alfred Pingoud. ChemBioChem, 2003

1,018 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/4f0c8eea24a586e208af0d84454c62b5e39019ae

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In Genetics

In Molecular Biology

Citations in the corpus

A versatile real-time PCR method to quantify bovine contamination in buffalo products (2013, methodology): “This technique can be used to quantify the initial DNA in a given sample (Kubista et al., 2006).”
Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s CT difference” formula (2006, background): “Generally, a PCR consists of four different kinetic stages [16, 17 ], which are basically similar to the wellknown bacterial growth kinetics as observed by Monod in 1949 [18].”
Molecular tools in rhizosphere microbiology—from single-cell to whole-community analysis (2009, methodology): “The TaqMan probe format relies on the specific binding of a hybridization probe to the target sequence and the cleavage of the probe by the endonuclease activity of the Taq DNA polymerase (Heid et al. 1996; Wilhelm and Pingoud 2003).”
Nucleic acid-based approaches to investigate microbial-related cheese quality defects (2012, methodology): “Fluorescence is then recorded vs. the number of cycles needed to reach saturation and from this the cycle threshold (Ct) is calculated (Bustin, 2005; Kubista et al., 2006; Postollec et al., 2011b).”
Diffusion maps for high-dimensional single-cell analysis of differentiation data (2015, background): “On the other hand, with the advent of new technologies, such as single-cell qPCR (Wilhelm and Pingoud, 2003) or RNA-Seq (Chu and Corey, 2012) and flow or mass cytometry (Bandura et al.”
How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments (2015, neutral): “Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard? Expert Rev Mol Diagn 2009;9(2):187–97.”
Diagnostic and prognostic implications of microRNA profiling in prostate carcinoma (2009, methodology): “GenEX software (MultiD Analyses AB, Göteborg, Sweden) was used to analyze and normalize the RT-qPCR data.(21) This program allows the correction of PCR efficiencies, the compensation for differences between runs by normalizing with interplate calibrators and the normalization with endogenous referen”
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. (2009, background): “The fluorescence-based quantitative real-time PCR (qPCR)15 (1–3), with its capacity to detect and measureminuteamountsofnucleicacidsinawiderangeof samples from numerous sources, is the enabling technology par excellence of molecular diagnostics, life sciences, agriculture, and medicine (4, 5 ). Its”
PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification (2009, background): “QPCR ampliﬁcation plots provide information for relative quantiﬁcation between samples and on the amount of initial DNA template (24–26).”