PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification
By Athanasia Spandidos, Xiaowei Wang, Huajun Wang, Brian Seed. Nucleic Acids Res., 2009
925 citations recorded by the source corpus.
PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306 800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as Tm, location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. There are several ways to search for primers for the gene(s) of interest, such as by: GenBank accession number, NCBI protein accession number, NCBI gene ID, PrimerBank ID, NCBI gene symbol or gene description (keyword). In all, 26 855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/68bbd19a25298958f93bf90da1b1519c5bde50c3
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
- Huajun Wang
- Athanasia Spandidos
- Thomas L. Madden
- Xiaowei Wang
- Brian Seed
- Douglas R. Green
- Jian Ye
- Steve Rozen
Citations in the corpus
- PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update (2011, background): “The PrimerBank resource and its experimentally validated 26 855 primer pairs covering most known mouse genes have previously been described (11–13).”
- Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction (2012, methodology): “As an example, we obtained the primers for the same PCR template as above (i.e., ZNF419 transcript variant 5 mRNA) from PrimerBank, which deposits many pre-computed gene-specific primers for detecting mRNA [16].”
- The transcription factor Myc controls metabolic reprogramming upon T lymphocyte activation (2011, methodology): “Primer sequences were obtained from PrimerBank (Spandidos et al., 2010) except for Gls1 and Gls2, which were manually designed.”
- Gut microbes promote colonic serotonin production through an effect of short‐chain fatty acids on enterochromaffin cells (2015, methodology): “All primer sequences used in this study were published previously and are described in Supplemental Table 1: mouse L32 (16, 17), Tph1 (18), Chga (chromogranin A) (19), andMaoa (monoamine oxidase A) (20), as well as human ACTB (21) and TPH1 (22); primer sequences for mouse Slc6a4 [serotonin transport”