Survival of Ca-alginate microencapsulated Bifidobacterium spp. in milk and simulated gastrointestinal conditions

By L. Hansen, P. Allan-Wojtas, Y-L Jin, A. Paulson. 2002

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Microencapsulation of probiotics for gastrointestinal delivery. (2012, methodology): “Various studies have been carried out on a variety of probiotic bacteria using both the extrusion [39, 49, 68, 69] and the emulsion[16, 69-73] methods, with varied success.”
PRINCIPLES AND METHODS OF MICROENCAPSULATION OF PROBIOTIC MICROORGANISMS (2007, unknown): “It should be noted that increasing the beads diameter to more than the particular limit (regarding type of capsule and microorganism) has been proved to have no significant effect on the viability of the cells (Truelstrup-Hansen et al., 2002).”
The influence of multi stage alginate coating on survivability of potential probiotic bacteria in simulated gastric and intestinal juice (2009, background): “This agrees with Truelstrup Hansen et al. (2002) reported that very large calcium alginate beads (>1 mm) cause a coarseness of texture in live microbial feed supplements. Moreover, Koo, Cho, Huh, Baek, and Park (2001) and Annan, Borza, and Truelstrup Hansen (2008) reported that the shape and size of”
Microencapsulation: An overview on concepts, methods, properties and applications in foods (2021, unknown): “The bacteria containing capsules were found to be more porous as compared to the core free capsules, hence attributing to the fact that the bacteria interfered with the formation of cross linking of the alginate with calcium chloride, as described by Truelstrup Hansen et al. (2002) and Sultana et al”