Rapid cloning of high-affinity human monoclonal antibodies against influenza virus

By J. Wrammert, Kenneth Smith, Joseph I. Miller, William A. Langley, K. Kokko, Christian Larsen, Nai-Ying Zheng, I. Mays, Lori Garman, Christina Helms and 5 more. Nature, 2008

1,029 citations recorded by the source corpus.

Subfields: Epidemiology, Immunology

View Paper: https://www.semanticscholar.org/paper/4af23a5b2d097ccd38e1ae1a989729b27e0b2aac

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Citations in the corpus

Generation of stable monoclonal antibody-producing BCR+ human memory B cells by genetic programming (2009, result): “The fact that rare B cells can easily be cloned compares our method favorably with a recently described single-cell cloning approach used to isolate influenza-specific antibodie”
From Vaccines to Memory and Back (2010, positive): “…followed by selection with functional assays (Traggiai et al., 2004), or by isolation of antigen-binding memory B cells or of total plasma cells followed by rescue of the specific antibodies by single cell PCR and expression in heterologous systems (Scheid et al., 2009; Wrammert et al., 2008).”
Standardizing immunophenotyping for the Human Immunology Project (2012, background): “Plasmablasts — the activated (CD38 + ) subset of antibody-secreting B cells — have been shown to increase in number post-vaccinatio”
Induction of ICOS+CXCR3+CXCR5+ TH Cells Correlates with Antibody Responses to Influenza Vaccination (2013, background): “Nonetheless, it is intriguing that the emergence of plasmablasts and ICOSCXCR3CXCR5CD4 T cells in blood peaks at the same time after influenza vaccination (at day 7) (33).”
Induction of broadly cross-reactive antibody responses to the influenza HA stem region following H5N1 vaccination in humans (2014, methodology): “HA-specific ELISA, as well as HAI (done with the chimeric H9N3 virus) were performed as previously described (40).”
Systems Biology of Seasonal Influenza Vaccination in Humans (2011, methodology): “Since the frequencies of plasmablasts return to barely detectable numbers by day 14 post-vaccination 15 , this correlation suggests that the later antibody response is associated with early circulation of plasmablasts in the blood of vaccinees 3 .”
Heterosubtypic Neutralizing Monoclonal Antibodies Cross-Protective against H5N1 and H1N1 Recovered from Human IgM+ Memory B Cells (2008, background): “However it has long been known that the immune response against influenza virus is highly restricted [15], as borne out by a recent cloning study [16], and focused on subtype and strain specific epitopes [17,18].”
Rapid profiling of RSV antibody repertoires from the memory B cells of naturally infected adult donors (2016, positive): “This result is in stark contrast to the relatively restricted repertoires observed in HIVinfected patients (31) or in healthy donors after influenza vaccination (32).”
Antibody landscapes after influenza virus infection or vaccination (2014, neutral): “The presence of higher preexposure static titers, but not higher dynamic responses, to the first infecting strain may explain seemingly contradictory reports whereby cross-sectional studies have tended to describe a serological bias supportive of the original antigenic sin dogma (5, 11) while invest”