Three-dimensional culture models of normal and malignant breast epithelial cells

By Genee Y. Lee, P. Kenny, Eva H. Lee, Mina J. Bissell. Nature Methods, 2007

1,313 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Oncology, Surgery

View Paper: https://www.semanticscholar.org/paper/4675d41fa55ed9009af359c6b51cb5470a34d13f

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Citations in the corpus

The morphologies of breast cancer cell lines in three‐dimensional assays correlate with their profiles of gene expression (2007, methodology): “…mammoplasty or breast tumors were cultured in a 3D culture assay in which cells are seeded singly on top of a thin gel comprised of laminin-rich extracellular matrix (Lee et al., 2007; Park et al., 2006), overlaid with medium containing a small amount of additional lrECM and analyzed after 4 days.”
Collective cell migration in morphogenesis, regeneration and cancer (2009, background): “, or by implanting multicellular spheroids that generate horizontal invasions into a 3D ECM cultur”
Engineering tumors with 3D scaffolds (2007, methodology): “Culture within this system recreated growth profiles similar to those detected in vivo , whereas tumor cells cultured in standard 3D Matrigel cultur”
Bioengineered 3D platform to explore cell-ECM interactions and drug resistance of epithelial ovarian cancer cells. (2010, background): “Contrary to studies using ‘overlay 3D cultures’ [22,31,51] or ‘3D on top assays’ [17], we used 3D cultures where cells were embedded within an anisotropic biomimetic hydrogel formed from liquid precursor solutions containing homogenously dispersed single EOC cells.”
In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract (2010, methodology): “The basement membrane extract, sold commercially as Matrigel, Cultrex and EHS extract, has been used extensively as a cell culture substratum for three-dimensional cultures to promote cell differentiatio”
A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity. (2018, positive): “Inspired by three-dimensional cell culture systems of the mammary epithelium (Lee et al., 2007; Shaw et al., 2004), we established culture protocols that allow the generation and longterm expansion of three-dimensional epithelial organoids (Sato and Clevers, 2015; Sato et al., 2009).”
Three-dimensional bioprinting of thick vascularized tissues (2016, background): “T ability to manufacture human tissues that replicate the essential spatial (1), mechanochemical (2, 3), and temporal aspects of biological tissues (4) would enable myriad applications, including 3D cell culture (5), drug screening (6, 7), disease modeling (8), and tissue repair and regeneration (9,”
Micropatterning as a tool to decipher cell morphogenesis and functions (2010, background): “When cells are placed on top of a soft matrix, without being fully immersed into it, they can form acini, suggesting that rigidity is more important than 3D encapsulation (Guo et al., 2008; Lee et al., 2007).”
3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained (2016, methodology): “(5) The final volume is automatically estimated by counting the voxels bound by the surface and using the x-y pixel resolution coefficient to convert voxels to μ M3.”