Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo

By A. Nimmerjahn, F. Kirchhoff, J. Kerr, Fritjof Helmchen. Nature Methods, 2004

821 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Developmental Neuroscience, Neurology

View Paper: https://www.semanticscholar.org/paper/44912616b9f578a298cd305a80a13a431392b20e

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In Cellular and Molecular Neuroscience

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Citations in the corpus

Broadly tuned response properties of diverse inhibitory neuron subtypes in mouse visual cortex (2010, neutral): “The injection solution also contained 60 mM sulforhodamine-101 (Invitrogen) to label cortical astrocytes (Nimmerjahn et al., 2004).”
Critical role of soluble amyloid-β for early hippocampal hyperactivity in a mouse model of Alzheimer’s disease (2012, methodology): “Astrocytes were excluded from the analysis based on their selective staining by sulforhodamine 101 (46) and their specific morphology.”
Automated analysis of cellular signals from large-scale calcium imaging data. (2009, methodology): “Targeted bulk-loading of cerebellar or neocortical tissue with the fluorescent Ca-indicator Oregon green 488 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate-1 acetoxymethyl (OGB-1-AM; Molecular Probes) was performed as described (Sullivan et al., 2005; Nimmerjahn et al., 2004; Nimmerjahn et al.”
Synaptic Islands Defined by the Territory of a Single Astrocyte (2007, background): “Cortical astrocytes are coupled by gap junctions (Nimmerjahn et al., 2004), raising the potential for communication between synaptic nodes supported by diffusion of molecules between neighboring astrocytes through these structures.”
Physiology of Astroglia. (2018, neutral): “Another gliophilic fluorescent probe that received much popularity in the imaging experiments is a cationic dye sulforhodamine 101 and its analogs sulforhodamine B or G (1224).”
Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain (2011, positive): “The Sca l e technique can also be applied effectively to chemically stained samples, as we found with transcardial perfusion of Texas Red–lectin to label blood vessels before fixation or in the future with in vivo labeling of astrocytes with Texas”
Astrocytic Ca2+ signaling evoked by sensory stimulation in vivo (2006, methodology): “Results Ca 2+ signaling events are rare in anesthetized mice The exposed barrel cortex of adult mice was loaded with both the Ca 2+ indicator fluo-4 AM (acetoxymethyl ester of fluo-4), and the astrocyte-specific fluorescent indicator sulforhodamine 101 (SR101”
An astrocytic basis of epilepsy (2005, methodology): “In initial experiments, fluo-4/AM was loaded concomitant with the astrocyte-specific marker sulforhodamine 101 (ref”
A disinhibitory microcircuit for associative fear learning in the auditory cortex (2011, methodology): “Full details of animals, materials and methods used for two-photon calcium imagin”