Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream.

By D. Ginzinger. Experimental Hematology, 2002

1,398 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/405d77199fc4b440c24b7f1a2ff0940cbedeb16d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. I. Mackay
2. Litao Yang
3. Vincenza Soleti ligorio
4. R. Hands
5. T. Yaseen
6. Andreas Nitsche
7. Stephen A. Bustin
8. Dabing Zhang

In Molecular Biology

1. T. Nolan
2. M. W. Pfaffl
3. I. Mackay
4. R. Hands
5. Stephen A. Bustin
6. Vladimir Benes
7. A. Ippolito
8. L. Schena

Citations in the corpus

• Real-Time PCR (2005, background): “These examples are but a few of the huge number of applications that have benefited from the introduction of real-time fluorescence-based PCR assays and that have contributed to the transformation of this technique from an experimental tool into the scientific mainstream.([30]) The advances in robot”
• A broad characterization of the transcriptional profile of the compatible tomato response to the plant parasitic root knot nematode Meloidogyne javanica (2005, background): “Infected:mock-inoculated expression ratios of target genes were determined as 2 ([average actin mockinoculated ) average gene of interest mock inoculated] ) [average actin infected ) average gene of interest infected]); average actin and gene of interest (either in nematode-infected or mock-inoculat”
• Guideline to reference gene selection for quantitative real-time PCR. (2004, methodology): “QPCR has become the most emerging method for quantification of mRNA transcription levels in recent years due to its outstanding accuracy, broad dynamic range, and sensitivity [2,3].”
• Quantitative real-time RT-PCR--a perspective. (2005, background): “Several factors have contributed to the transformation of this technology into a mainstream research tool: (i) as a homogeneous assay it avoids the need for post-PCR processing; (ii) a wide (>107-fold) dynamic range allows straightforward comparison between RNAs that differ widely in their abundance”
• Salivary Transcriptome Diagnostics for Oral Cancer Detection (2004, methodology): “The initial amount of cDNA/RNA of a particular template was extrapolated from the standard curve as described previously (21).”
• Quantification of mRNA using real-time RT-PCR (2006, methodology): “Although it has become the method of choice for the quantification of RN”
• Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators (2011, background): “The main advantages of this technique are high sensitivity, high specificity and broad quantification range (Bustin et al. 2005; Bustin and Nolan 2004; Gahon et al. 2004; Ginzinger 2002).”
• Real-time detection of Phytophthora nicotianae and P. citrophthorain citrus roots and soil (2004, methodology): “A substantial advantage of real-time PCR when compared with conventional PCR is that the early PCR cycles can be recorded and, apart from the plateau effect, very subtle changes in detection (i.e. changes in Ct) can be monitored, enabling quantitative analyses (Bell et al., 1999; Ginzinger, 2002).”
• Real‐time PCR (qPCR) primer design using free online software (2011, background): “TaqMan(1) (1) utilizes a probe which consists of an oligonucleotide sequence with a 50 fluorescent reporter molecule (F) and a 30 quencher dye (Q).”
• Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi (2004, methodology): “Several reviews on the use of real-time PCR in fields other than plant pathology have been published recently (Bustin, 2000, 2002; Didenko, 2001; Lyon, 2001; Schmittgen, 2001; Snider et al., 2001; Ahmed, 2002; Broude, 2002; Ginzinger, 2002).”
• Estimating number of transgene copies in transgenic rapeseed by real-time PCR assay withHMG I/Y as an endogenous reference gene (2004, methodology): “This technique offers major advantages over the other methods, including a large dynamic range of quantification, no post-PCR manipulations (greatly reducing the risk of carryover contamination), use of small amounts of starting material, and high-throughput capacity (Celi et al., 1994; De Preter et”