Real-time detection of Phytophthora nicotianae and P. citrophthorain citrus roots and soil

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Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi (2004, background): “citrophthora in citrus roots (Ippolito et al., 2004), Pyrenophora teres on infected barley seed (Bates et al.”
Development and application of a PCR‐based ‘molecular tool box’ for the identification of Phytophthora species damaging forests and natural ecosystems (2007, background): “…leaf samples, soil and water samples required a nested approach to achieve appropriate levels of sensitivity, not unexpectedly given that the concentration of Phytophthora in soil and water is probably nearly always under the detection limit of a single PCR amplification (Ippolito et al., 2004).”