Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity

By H. Christianson, Katrin J. Svensson, T. V. van Kuppevelt, Jin-ping Li, Mattias Belting. Proceedings of the National Academy of Sciences of the United States of America, 2013

835 citations recorded by the source corpus.

Subfields: Infectious Diseases, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3f3572aba6402adfe1731ffa3f469d6b002a457e

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Infectious Diseases

In Molecular Biology

Citations in the corpus

Quantitative and stoichiometric analysis of the microRNA content of exosomes (2014, background): “, proteoglycans) and internalized by recipient cells (8), resulting in transfer of the exosome contents.”
Exosomes in cancer: small particle, big player (2015, background): “Enzymatic depletion of cell-surface HSPG or pharmacological inhibition of endogenous proteoglycan biosynthesis significantly attenuates exosome uptake [18].”
Exosome Theranostics: Biology and Translational Medicine (2018, background): “In addition, some studies have shown that heparan sulfate proteoglycans (HSPGs) are used as internalizing receptors by cancer cell-derived exosomes [3].”
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper (2017, background): “uptake.[241,242] Furthermore, it was recently demonstrated that various isoforms of integrins present on tumour-derived EVs influence the uptake of EVs in an organ-specific manner.”
Routes and mechanisms of extracellular vesicle uptake (2014, background): “Other control experiments, such as incubation of cells with excess unlabelled EVs and direct measurement of the rate of transfer of fluorescence between EVs also support the idea that the increased fluorescence in recipient cells is due to specific uptake of EVs rather than non-specific dye leaching”
Exosome Uptake through Clathrin-mediated Endocytosis and Macropinocytosis and Mediating miR-21 Delivery* (2014, background): “Heparan sulfate proteoglycans (HSPGs) were reported to function as receptors of glioblastoma cell-derived exosomes (32).”
SORTING IT OUT: REGULATION OF EXOSOME LOADING (2014, background): “Experiments with exogenous HS, pan-PG deficient cells, and pharmacological inhibitors of HSPG have shown that cell-surface HS is required for efficient exosome uptake [105].”
Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis (2019, background): “The presence of cytosolic and plasma membrane proteins can be understood based on the biogenesis of MVs, similarly, it can be understood that proteins specifically associated with different organelles such as the mitochondria, Golgi apparatus, nucleus, and endoplasmic reticulum should be depleted in”