Quantitative and stoichiometric analysis of the microRNA content of exosomes

By J. Chevillet, Q. Kang, I. K. Ruf, H. Briggs, Lucia Vojtech, S. Hughes, Heather H. Cheng, Jason D. Arroyo, Emily Meredith, Emily N. Gallichotte and 7 more. Proceedings of the National Academy of Sciences of the United States of America, 2014

1,020 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/874b814967988743d1b6ad87b6790a065977cc9a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. Raghu Kalluri
2. E. Batrakova
3. M. Kim
4. Valerie S. LeBleu
5. M. Wood
6. P. Vader
7. Edit I. Buzás
8. S. Bhattacharyya

In Molecular Biology

1. Raghu Kalluri
2. E. Batrakova
3. Valerie S. LeBleu
4. M. Kim
5. P. Vader
6. M. Wood
7. Imre Mäger
8. Edit I. Buzás

Citations in the corpus

• Effect of exosomal miRNA on cancer biology and clinical applications (2018, background): “At the same time, miR-21 and miR-29a act in NSCLC tumor growth and metastasis [41], and miR-126 promotes the Hematological malignancies metastasis [90].”
• The biology, function, and biomedical applications of exosomes (2020, background): “Although exosomal cargo analyses require large pools of purified exosomes, not all exosomes contain a similar abundance of a given cargo, as observed, for example, with miRNA exosomal cargo (22).”
• Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper (2017, background): “RT-qPCR is sensitive to DNA contamination, though this can be minimised by good experimental practice, such as running a gel to verify a single amplicon of the expected size and designing primers over exon-exon junctions in the case of mRNA.”
• Exosome and Exosomal MicroRNA: Trafficking, Sorting, and Function (2015, background): “They found that, on average, most exosomes did not harbor many copies of miRNA molecule [77].”
• Using exosomes, naturally-equipped nanocarriers, for drug delivery (2015, background): “” In addition, exosomes themselves compose a fairly heterogeneous population in terms of their biochemical composition, the source (different cell lines or patient samples) often dictates exosome phenotype [23]; this has critical implications for the use of exosomes in the clinic.”
• Plant exosome like nanovesicles: Emerging therapeutics and drug delivery nanoplatforms. (2020, methodology): “The characteristic intraluminal vesicles (ILVs), which actively and selectively incorporate RNAs, including mRNAs, microRNAs (miRNAs), and other non-coding RNAs along with lipids and DNA, dwell inside MVBs, which are ultimately integrated with plasmalemma to release PELNVs.23 PELNVs, being an integr”
• Extracellular Vesicle Heterogeneity: Subpopulations, Isolation Techniques, and Diverse Functions in Cancer Progression (2018, background): “For example, analyses of miRNA stoichiometry in EVs reveal that most of the EVs released by cells will not contain biologically significant numbers of miRNA copies (44).”
• Extracellular miRNAs: From Biomarkers to Mediators of Physiology and Disease (2019, background): “Indeed, considering the relatively small amounts of miRNAs carried by EVs in circulation in comparison to the amounts found within the cells (Chevillet et al., 2014), it seems likely that there is some mechanism of selective delivery, such that miRNAs which enter their target cells are able to promo”